Universidad Peruana Cayetano Heredia

An updated insight into the Sialotranscriptome of Triatoma infestans: developmental stage and geographic variations

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dc.contributor.author Schwarz, Alexandra
dc.contributor.author Medrano-Mercado, Nora
dc.contributor.author Schaub, Günter A.
dc.contributor.author Struchiner, Claudio J.
dc.contributor.author Bargues, M. Dolores
dc.contributor.author Levy, Michael Z.
dc.contributor.author Ribeiro, José M. C.
dc.date.accessioned 2020-06-10T18:12:17Z
dc.date.available 2020-06-10T18:12:17Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/20.500.12866/8095
dc.description.abstract BACKGROUND: Triatoma infestans is the main vector of Chagas disease in South America. As in all hematophagous arthropods, its saliva contains a complex cocktail that assists blood feeding by preventing platelet aggregation and blood clotting and promoting vasodilation. These salivary components can be immunologically recognized by their vector's hosts and targeted with antibodies that might disrupt blood feeding. These antibodies can be used to detect vector exposure using immunoassays. Antibodies may also contribute to the fast evolution of the salivary cocktail. METHODOLOGY: Salivary gland cDNA libraries from nymphal and adult T. infestans of breeding colonies originating from different locations (Argentina, Chile, Peru and Bolivia), and cDNA libraries originating from F1 populations of Bolivia, were sequenced using Illumina technology. Coding sequences (CDS) were extracted from the assembled reads, the numbers of reads mapped to these CDS, sequences were functionally annotated and polymorphisms determined. MAIN FINDINGS/SIGNIFICANCE: Over five thousand CDS, mostly full length or near full length, were publicly deposited on GenBank. Transcripts that were over 10-fold overexpressed from different geographical regions, or from different developmental stages were identified. Polymorphisms were mapped to derived coding sequences, and found to vary between developmental instars and geographic origin of the biological material. This expanded sialome database from T. infestans should be of assistance in future proteomic work attempting to identify salivary proteins that might be used as epidemiological markers of vector exposure, or proteins of pharmacological interest. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Gene Library en_US
dc.subject Animals en_US
dc.subject Saliva/chemistry en_US
dc.subject Salivary Proteins and Peptides/genetics/metabolism en_US
dc.subject South America en_US
dc.subject Transcriptome/genetics en_US
dc.subject Triatoma/genetics/metabolism en_US
dc.title An updated insight into the Sialotranscriptome of Triatoma infestans: developmental stage and geographic variations en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0003372
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1935-2735


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