Universidad Peruana Cayetano Heredia

In vitro evaluation of a soluble Leishmania promastigote surface antigen as a potential vaccine candidate against human leishmaniasis

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dc.contributor.author Chamakh-Ayari, Rym
dc.contributor.author Bras-Gonçalves, Rachel
dc.contributor.author Bahi-Jaber, Narges
dc.contributor.author Petitdidier, Elodie
dc.contributor.author Markikou-Ouni, Wafa
dc.contributor.author Aoun, Karim
dc.contributor.author Moreno, Javier
dc.contributor.author Carrillo, Eugenia
dc.contributor.author Salotra, Poonam
dc.contributor.author Kaushal, Himanshu
dc.contributor.author Negi, Narender Singh
dc.contributor.author Arévalo Zelada, Jorge Luis
dc.contributor.author Falconi-Agapito, Francesca
dc.contributor.author Privat, Angela
dc.contributor.author Cruz, Maria
dc.contributor.author Pagniez, Julie
dc.contributor.author Papierok, Gérard-Marie
dc.contributor.author Rhouma, Faten Bel Haj
dc.contributor.author Torres, Pilar
dc.contributor.author Lemesre, Jean-Loup
dc.contributor.author Chenik, Mehdi
dc.contributor.author Meddeb-Garnaoui, Amel
dc.date.accessioned 2020-06-10T18:12:17Z
dc.date.available 2020-06-10T18:12:17Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/20.500.12866/8097
dc.description.abstract PSA (Promastigote Surface Antigen) belongs to a family of membrane-bound and secreted proteins present in several Leishmania (L.) species. PSA is recognized by human Th1 cells and provides a high degree of protection in vaccinated mice. We evaluated humoral and cellular immune responses induced by a L. amazonensis PSA protein (LaPSA-38S) produced in a L. tarentolae expression system. This was done in individuals cured of cutaneous leishmaniasis due to L. major (CCLm) or L. braziliensis (CCLb) or visceral leishmaniasis due to L. donovani (CVLd) and in healthy individuals. Healthy individuals were subdivided into immune (HHR-Lm and HHR-Li: Healthy High Responders living in an endemic area for L. major or L. infantum infection) or non immune/naive individuals (HLR: Healthy Low Responders), depending on whether they produce high or low levels of IFN-γ in response to Leishmania soluble antigen. Low levels of total IgG antibodies to LaPSA-38S were detected in sera from the studied groups. Interestingly, LaPSA-38S induced specific and significant levels of IFN-γ, granzyme B and IL-10 in CCLm, HHR-Lm and HHR-Li groups, with HHR-Li group producing TNF-α in more. No significant cytokine response was observed in individuals immune to L. braziliensis or L. donovani infection. Phenotypic analysis showed a significant increase in CD4+ T cells producing IFN-γ after LaPSA-38S stimulation, in CCLm. A high positive correlation was observed between the percentage of IFN-γ-producing CD4+ T cells and the released IFN-γ. We showed that the LaPSA-38S protein was able to induce a mixed Th1 and Th2/Treg cytokine response in individuals with immunity to L. major or L. infantum infection indicating that it may be exploited as a vaccine candidate. We also showed, to our knowledge for the first time, the capacity of Leishmania PSA protein to induce granzyme B production in humans with immunity to L. major and L. infantum infection. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS ONE
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Adaptive Immunity en_US
dc.subject Animals en_US
dc.subject Antigens, Protozoan/biosynthesis/chemistry/immunology en_US
dc.subject Antigens, Surface/biosynthesis/chemistry/immunology en_US
dc.subject Granzymes/blood en_US
dc.subject Humans en_US
dc.subject Immunity, Humoral en_US
dc.subject Interferon-gamma/blood en_US
dc.subject Interleukin-10/blood en_US
dc.subject Leishmania/immunology en_US
dc.subject Leishmaniasis/blood/immunology/prevention & control en_US
dc.subject Mice en_US
dc.subject Phenotype en_US
dc.subject Protozoan Vaccines/biosynthesis/chemistry/immunology en_US
dc.subject Solubility en_US
dc.subject Tumor Necrosis Factor-alpha/blood en_US
dc.title In vitro evaluation of a soluble Leishmania promastigote surface antigen as a potential vaccine candidate against human leishmaniasis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pone.0092708
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 1932-6203


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