DSpace Repository

Evans blue staining reveals vascular leakage associated with focal areas of host-parasite interaction in brains of pigs infected with Taenia solium

Show simple item record

dc.contributor.author Marzal, Miguel
dc.contributor.author Guerra-Giraldez, Cristina
dc.contributor.author Paredes, Adriana
dc.contributor.author Cangalaya, Carla
dc.contributor.author Rivera, Andrea
dc.contributor.author Gonzalez Zariquiey, Armando Emiliano
dc.contributor.author Mahanty, Siddhartha
dc.contributor.author García Lescano, Héctor Hugo
dc.contributor.author Nash, Theodore E.
dc.date.accessioned 2020-06-10T18:12:17Z
dc.date.available 2020-06-10T18:12:17Z
dc.date.issued 2014
dc.identifier.uri https://hdl.handle.net/20.500.12866/8103
dc.description.abstract Cysticidal drug treatment of viable Taenia solium brain parenchymal cysts leads to an acute pericystic host inflammatory response and blood brain barrier breakdown (BBB), commonly resulting in seizures. Naturally infected pigs, untreated or treated one time with praziquantel were sacrificed at 48 hr and 120 hr following the injection of Evans blue (EB) to assess the effect of treatment on larval parasites and surrounding tissue. Examination of harvested non encapsulated muscle cysts unexpectedly revealed one or more small, focal round region(s) of Evans blue dye infiltration (REBI) on the surface of otherwise non dye-stained muscle cysts. Histopathological analysis of REBI revealed focal areas of eosinophil-rich inflammatory infiltrates that migrated from the capsule into the tegument and internal structures of the parasite. In addition some encapsulated brain cysts, in which the presence of REBI could not be directly assessed, showed histopathology identical to that of the REBI. Muscle cysts with REBI were more frequent in pigs that had received praziquantel (6.6% of 3736 cysts; n = 6 pigs) than in those that were untreated (0.2% of 3172 cysts; n = 2 pigs). Similar results were found in the brain, where 20.7% of 29 cysts showed histopathology identical to muscle REBI cysts in praziquantel-treated pigs compared to the 4.3% of 47 cysts in untreated pigs. Closer examination of REBI infiltrates showed that EB was taken up only by eosinophils, a major component of the cellular infiltrates, which likely explains persistence of EB in the REBI. REBI likely represent early damaging host responses to T. solium cysts and highlight the focal nature of this initial host response and the importance of eosinophils at sites of host-parasite interaction. These findings suggest new avenues for immunomodulation to reduce inflammatory side effects of anthelmintic therapy. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS ONE
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Host-Parasite Interactions en_US
dc.subject Animals en_US
dc.subject Brain/blood supply/parasitology/pathology en_US
dc.subject Evans Blue/pharmacokinetics en_US
dc.subject Praziquantel/therapeutic use en_US
dc.subject Swine en_US
dc.subject Taenia solium/pathogenicity en_US
dc.subject Taeniasis/drug therapy/veterinary en_US
dc.title Evans blue staining reveals vascular leakage associated with focal areas of host-parasite interaction in brains of pigs infected with Taenia solium en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pone.0097321
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#4.03.01
dc.relation.issn 1932-6203


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Except where otherwise noted, this item's license is described as info:eu-repo/semantics/restrictedAccess

Search DSpace


Browse

My Account

Statistics