Semen parameters of fertile Guinea pigs (Cavia porcellus) collected by transrectal electroejaculation

Benavides, F.; Echevarría Curee, Luisa Inés; Kennedy, C.; Sutovsky, P.; López, V.

dc.contributor.author	Benavides, F.
dc.contributor.author	Sutovsky, P.
dc.contributor.author	López, V.
dc.contributor.author	Kennedy, C.
dc.contributor.author	Echevarría Curee, Luisa Inés
dc.date.accessioned	2020-07-14T00:00:56Z
dc.date.available	2020-07-14T00:00:56Z
dc.date.issued	2020
dc.identifier.uri	https://hdl.handle.net/20.500.12866/8231
dc.description.abstract	The guinea pig, as a livestock species, is still developing and growing throughout Peru and neighboring countries, as reflected by its increasing export since 2000. However, the selection of proven fertile males is tedious due to the absence of seminal parameter standards and the lack of safe semen collection techniques. Thus, pregnancy detection or live births are required for males’ selection. The purpose of this study was to describe the qualitative and quantitative semen parameters of fertile guinea pig males, to set reference values, and to validate a novel electroejaculation technique for the species. Semen was collected at weekly intervals from sixteen fertile males. Four transrectal electroejaculations were performed per male with 95% successful collections, yielding 39 viable semen samples. Seminal characteristics were as follows: pH 7.0 ± 0.13; ejaculate volume 0.67 ± 0.55 mL; sperm motility 90.81 ± 6.64%; sperm concentration 36.7 ± 28.41 × 106 sperm/mL; sperm count 20.09 ± 17.56 × 106 sperm/ejaculate; percentage of abnormal morphology 18.26 ± 8.52%; and percentage ubiquitinated spermatozoa 5.57 ± 6.28%. These values will serve as a reference to detect best breeding and infertile males rapidly. The described techniques are reproducible by commercial producers.	en_US
dc.language.iso	eng
dc.publisher	MDPI
dc.relation.ispartofseries	Animals
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	prevalence	en_US
dc.subject	Article	en_US
dc.subject	controlled study	en_US
dc.subject	male	en_US
dc.subject	nonhuman	en_US
dc.subject	adult	en_US
dc.subject	animal experiment	en_US
dc.subject	microscopy	en_US
dc.subject	feces analysis	en_US
dc.subject	male infertility	en_US
dc.subject	protein	en_US
dc.subject	flow cytometry	en_US
dc.subject	acrosome	en_US
dc.subject	Cavia porcellus	en_US
dc.subject	ejaculation	en_US
dc.subject	fertility	en_US
dc.subject	fertilization	en_US
dc.subject	guinea pig	en_US
dc.subject	insemination	en_US
dc.subject	semen analysis	en_US
dc.subject	seminal plasma	en_US
dc.subject	sperm preservation	en_US
dc.subject	sperm quality	en_US
dc.subject	spermatozoon	en_US
dc.subject	spermatozoon count	en_US
dc.subject	spermatozoon density	en_US
dc.subject	spermatozoon motility	en_US
dc.subject	transrectal electroejaculation	en_US
dc.subject	ubiquitin	en_US
dc.title	Semen parameters of fertile Guinea pigs (Cavia porcellus) collected by transrectal electroejaculation	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.3390/ani10050767
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#4.03.00
dc.relation.issn	2076-2615