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Semen parameters of fertile Guinea pigs (Cavia porcellus) collected by transrectal electroejaculation

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dc.contributor.author Benavides, F.
dc.contributor.author Sutovsky, P.
dc.contributor.author López, V.
dc.contributor.author Kennedy, C.
dc.contributor.author Echevarría, L.
dc.date.accessioned 2020-07-14T00:00:56Z
dc.date.available 2020-07-14T00:00:56Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/20.500.12866/8231
dc.description.abstract The guinea pig, as a livestock species, is still developing and growing throughout Peru and neighboring countries, as reflected by its increasing export since 2000. However, the selection of proven fertile males is tedious due to the absence of seminal parameter standards and the lack of safe semen collection techniques. Thus, pregnancy detection or live births are required for males’ selection. The purpose of this study was to describe the qualitative and quantitative semen parameters of fertile guinea pig males, to set reference values, and to validate a novel electroejaculation technique for the species. Semen was collected at weekly intervals from sixteen fertile males. Four transrectal electroejaculations were performed per male with 95% successful collections, yielding 39 viable semen samples. Seminal characteristics were as follows: pH 7.0 ± 0.13; ejaculate volume 0.67 ± 0.55 mL; sperm motility 90.81 ± 6.64%; sperm concentration 36.7 ± 28.41 × 106 sperm/mL; sperm count 20.09 ± 17.56 × 106 sperm/ejaculate; percentage of abnormal morphology 18.26 ± 8.52%; and percentage ubiquitinated spermatozoa 5.57 ± 6.28%. These values will serve as a reference to detect best breeding and infertile males rapidly. The described techniques are reproducible by commercial producers. en_US
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartof urn:issn:2076-2615
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject prevalence en_US
dc.subject Article en_US
dc.subject controlled study en_US
dc.subject male en_US
dc.subject nonhuman en_US
dc.subject adult en_US
dc.subject animal experiment en_US
dc.subject microscopy en_US
dc.subject feces analysis en_US
dc.subject male infertility en_US
dc.subject protein en_US
dc.subject flow cytometry en_US
dc.subject acrosome en_US
dc.subject Cavia porcellus en_US
dc.subject ejaculation en_US
dc.subject fertility en_US
dc.subject fertilization en_US
dc.subject guinea pig en_US
dc.subject insemination en_US
dc.subject semen analysis en_US
dc.subject seminal plasma en_US
dc.subject sperm preservation en_US
dc.subject sperm quality en_US
dc.subject spermatozoon en_US
dc.subject spermatozoon count en_US
dc.subject spermatozoon density en_US
dc.subject spermatozoon motility en_US
dc.subject transrectal electroejaculation en_US
dc.subject ubiquitin en_US
dc.title Semen parameters of fertile Guinea pigs (Cavia porcellus) collected by transrectal electroejaculation en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3390/ani10050767
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#4.03.00


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