ZEITLUPE facilitates the rhythmic movements of Nicotiana attenuata flowers

Li, Ran; Yon, Felipe; Schäfer, Martin; Baldwin, Ian T.; Kim, Sang-Gyu; Cortés Llorca, Lucas; Halitschke, Rayko; Robert, Christelle A. M.

dc.contributor.author	Cortés Llorca, Lucas
dc.contributor.author	Li, Ran
dc.contributor.author	Yon, Felipe
dc.contributor.author	Schäfer, Martin
dc.contributor.author	Halitschke, Rayko
dc.contributor.author	Robert, Christelle A. M.
dc.contributor.author	Kim, Sang-Gyu
dc.contributor.author	Baldwin, Ian T.
dc.date.accessioned	2020-07-14T00:01:06Z
dc.date.available	2020-07-14T00:01:06Z
dc.date.issued	2020
dc.identifier.uri	https://hdl.handle.net/20.500.12866/8270
dc.description.abstract	Circadian organ movements are ubiquitous in plants. These rhythmic outputs are thought to be regulated by the circadian clock and auxin signalling, but the underlying mechanisms have not been clarified. Flowers of Nicotiana attenuata change their orientation during the daytime through a 140° arc to balance the need for pollinators and the protection of their reproductive organs. This rhythmic trait is under the control of the circadian clock and results from bending and re‐straightening movements of the pedicel, stems that connect flowers to the inflorescence. Using an explant system that allowed pedicel growth and curvature responses to be characterized with high spatial and temporal resolution, we demonstrated that this movement is organ autonomous and mediated by auxin. Changes in the growth curvature of the pedicel are accompanied by an auxin gradient and dorsiventral asymmetry in auxin‐dependent transcriptional responses; application of auxin transport inhibitors influenced the normal movements of this organ. Silencing the expression of the circadian clock component ZEITLUPE (ZTL) arrested changes in the growth curvature of the pedicel and altered auxin signalling and responses. IAA19‐like, an Aux/IAA transcriptional repressor that is circadian regulated and differentially expressed between opposite tissues of the pedicel, and therefore possibly involved in the regulation of changes in organ curvature, physically interacted with ZTL. Together, these results are consistent with a direct link between the circadian clock and the auxin signalling pathway in the regulation of this rhythmic floral movement.	en_US
dc.language.iso	eng
dc.publisher	Wiley
dc.relation.ispartofseries	Plant Journal
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject	auxin signalling	en_US
dc.subject	circadian clock	en_US
dc.subject	Nicotiana attenuata	en_US
dc.subject	plant movement	en_US
dc.title	ZEITLUPE facilitates the rhythmic movements of Nicotiana attenuata flowers	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.1111/tpj.14732
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#1.06.10
dc.relation.issn	1365-313X