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Lower Body Weight in Rats Under Hypobaric Hypoxia Exposure Would Lead to Reduced Right Ventricular Hypertrophy and Increased AMPK Activation

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dc.contributor.author Flores, K.
dc.contributor.author Siques, P.
dc.contributor.author Brito, J.
dc.contributor.author Ordenes, S.
dc.contributor.author Arriaza, K.
dc.contributor.author Pena, E.
dc.contributor.author León-Velarde, F.
dc.contributor.author López, R.
dc.contributor.author López de Pablo, Á.L.
dc.contributor.author Arribas, S.
dc.date.accessioned 2020-07-14T00:01:10Z
dc.date.available 2020-07-14T00:01:10Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/20.500.12866/8290
dc.description.abstract Background: Both chronic hypoxia (CH) and long-term chronic intermittent hypoxia (CIH) exposure lead to right ventricular hypertrophy (RVH). Weight loss is an effective intervention to improve cardiac function and energy metabolism in cardiac hypertrophy. Likewise, caloric restriction (CR) also plays an important role in this cardioprotection through AMPK activation. We aimed to determine the influence of body weight (BW) on RVH, AMPK and related variables by comparing rats exposed to both hypoxic conditions. Methods: Sixty male adult rats were separated into two groups (n = 30 per group) according to their previous diet: a caloric restriction (CR) group and an ad libitum (AL) group. Rats in both groups were randomly assigned to 3 groups: a normoxic group (NX, n = 10), a CIH group (2 days hypoxia/2 days normoxia; n = 10) and a CH group (n = 10). The CR group was previously fed 10 g daily, and the other was fed ad libitum. Rats were exposed to simulated hypobaric hypoxia in a hypobaric chamber set to 428 Torr (the equivalent pressure to that at an altitude of 4,600 m above sea level) for 30 days. Measurements included body weight; hematocrit; serum insulin; glycemia; the degree of RVH (Fulton’s index and histology); and AMPK, mTOR, and PP2C expression levels in the right ventricle determined by western blotting. Results: A lower degree of RVH, higher AMPK activation, and no activation of mTOR were found in the CR groups exposed to hypobaric hypoxia compared to the AL groups (p < 0.05). Additionally, decreased glycemia and serum insulin levels were observed. Interestingly, PP2C expression showed an increase in the AL groups but not in the CR groups (p < 0.05). Conclusion: Maintaining a low weight before and during exposure to high-altitude hypoxia, during either CH or CIH, could prevent a major degree of RVH. This cardioprotection would likely be due to the activation of AMPK. Thus, body weight is a factor that might contribute to RVH at high altitudes. en_US
dc.language.iso eng
dc.publisher Frontiers Media
dc.relation.ispartof urn:issn:1664-042X
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Article en_US
dc.subject controlled study en_US
dc.subject male en_US
dc.subject nonhuman en_US
dc.subject adult en_US
dc.subject food intake en_US
dc.subject rat en_US
dc.subject altitude disease en_US
dc.subject body weight loss en_US
dc.subject glucose en_US
dc.subject glucose blood level en_US
dc.subject protein expression en_US
dc.subject hematocrit en_US
dc.subject Western blotting en_US
dc.subject caloric restriction en_US
dc.subject chronic intermittent hypoxia en_US
dc.subject enzyme activation en_US
dc.subject Fulton index en_US
dc.subject heart protection en_US
dc.subject heart right ventricle hypertrophy en_US
dc.subject hydroxymethylglutaryl coenzyme A reductase kinase en_US
dc.subject insulin en_US
dc.subject insulin blood level en_US
dc.subject mammalian target of rapamycin en_US
dc.subject protein phosphatase 2c en_US
dc.title Lower Body Weight in Rats Under Hypobaric Hypoxia Exposure Would Lead to Reduced Right Ventricular Hypertrophy and Increased AMPK Activation en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3389/fphys.2020.00342
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.08


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