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Synthesis, characterization and bio-functionalization of magnetic nanoparticles to improve the diagnosis of tuberculosis

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dc.contributor.author León-Janampa, Nancy
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.contributor.author Shinkaruk, Svitlana
dc.contributor.author Quispe-Marcatoma, J.
dc.contributor.author Gutarra, Abel
dc.contributor.author Le Bourdon, Gwénaëlle
dc.contributor.author Gayot, Marion
dc.contributor.author Changanaqui, Katherina
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Fouquet, Eric
dc.contributor.author Sheen Cortavarria, Patricia
dc.contributor.author Szlosek, Magali
dc.date.accessioned 2020-07-14T00:02:31Z
dc.date.available 2020-07-14T00:02:31Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/20.500.12866/8327
dc.description.abstract Mycobacterium tuberculosis is the cause of one of the diseases with the highest mortality and morbidity rate in the Americas and in the world. In developing countries, the diagnosis of tuberculosis (TB) is based on baciloscopy and bacteriological cultures. The first method has a low sensitivity, and the second can take several weeks to reach a confirmatory diagnosis. The lack of a rapid diagnosis compromises the efforts to control this disease and favors the transmission of tuberculosis to the susceptible population. In this work, we present the synthesis, amine-silanization, characterization and bio-functionalization of magnetic nanoparticles (MNPs) to develop a sandwich ELISA to detect and concentrate antigens from M. tuberculosis. For this purpose, a recombinant mycobacterial heat shock protein Hsp16.3, which contributes to the persistence of TB, was cloned and expressed in the E. coli system. Polyclonal antibodies anti-Hsp16.3 were produced in a rabbit and in mice. Magnetic nanoparticles were synthesized by co-precipitation, amine-functionalized and characterized by several physical-chemical methods. The XRD, Mossbauer spectroscopy, zeta potential, TEM, and FTIR all proved the successful preparation of the MNPs showing a diffraction crystal diameter of 10.48 ± 2.56 nm, superficial net charge of : +23.57 ± 2.87 mV, characteristic patterns of magnetite and a structure similar to a sphere. Additionally, it showed a magnetization saturation of 37.06 emu.g-1. For the functionalization of nanoparticle surfaces with anti-Hsp16.3, the active ester method was used for bond formation, and parameters such as time of incubation, coupling agents ratio (EDC/NHS) and concentration as well as surface saturation level of amine-silanized MNPs (MNP@Si@NH2) were standardized. Finally, bio-functionalized MNPs were used to detect, fix and concentrate the recombinant antigen Hsp16.3 from M. tuberculosis in a sandwich ELISA-MNP assay. en_US
dc.language.iso eng
dc.publisher IOP Publishing
dc.relation.ispartofseries Nanotechnology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject magnetic nanoparticles en_US
dc.subject diagnosis en_US
dc.subject tuberculosis en_US
dc.title Synthesis, characterization and bio-functionalization of magnetic nanoparticles to improve the diagnosis of tuberculosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1088/1361-6528/ab6ab1
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#2.10.00
dc.relation.issn 1361-6528


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