DSpace Repository

Comparative characterization of Viperidae snake venoms from Peru reveals two compositional patterns of phospholipase A2 expression

Show simple item record

dc.contributor.author Lomonte, Bruno
dc.contributor.author Díaz, Cecilia
dc.contributor.author Chaves, Fernando
dc.contributor.author Fernández, Julián
dc.contributor.author Ruiz, Marco
dc.contributor.author Salas, María
dc.contributor.author Zavaleta, Alfonso
dc.contributor.author Calvete, Juan J.
dc.contributor.author Sasa, Mahmood
dc.date.accessioned 2020-07-14T00:02:31Z
dc.date.available 2020-07-14T00:02:31Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/20.500.12866/8328
dc.description.abstract Snake species within the Bothrops complex (sensu lato) are of medical relevance in Latin America, but knowledge on their venom characteristics is limited, or even unavailable, for some taxa. Perú harbors 17 species of pit vipers, within the genera Bothrops, Bothriechis, Bothrocophias, Porthidium, Crotalus, and Lachesis. This study compared the venoms of twelve species, through chromatographic and electrophoretic profiles, as well as proteolytic and phospholipase A2 (PLA2) activities. Also, proteomic profiles were analyzed for nine of the venoms using a shotgun approach. Results unveiled conspicuous differences in the expression of venom PLA2s among species, six of them presenting scarce levels as judged by RP-HPLC profiles. Since most species within the bothropoid lineage possess venoms with high to intermediate abundances of this protein family, our findings suggest the existence of a phenotypic duality in the expression of venom PLA2s within the Bothrops (sensu lato) complex. Bothrops barnetti and Bothrocophias andianus venoms, very scarce in PLA2s, were shown to lack significant myotoxic activity, highlighting that the observed variability in PLA2 expression bears toxicological correlations with effects attributed to these proteins. Finally, an attempt to identify phylogenetic relationships of bothropoid species from Perú presenting low- or high-PLA2 venom phenotypes showed an interspersed pattern, thus precluding a simple phylogenetic interpretation of this venom compositional dichotomy. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof urn:issn:2590-1710
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Phenotype en_US
dc.subject Bothropoid en_US
dc.subject Dichotomy en_US
dc.subject Phospholipase A2 en_US
dc.subject Snake venom en_US
dc.subject Viperidae en_US
dc.title Comparative characterization of Viperidae snake venoms from Peru reveals two compositional patterns of phospholipase A2 expression en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.toxcx.2020.100044
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.07


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

info:eu-repo/semantics/restrictedAccess Except where otherwise noted, this item's license is described as info:eu-repo/semantics/restrictedAccess

Search DSpace


Browse

My Account

Statistics