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Quantification of malaria antigens PfHRP2 and pLDH by quantitative suspension array technology in whole blood, dried blood spot and plasma

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dc.contributor.author Martiáñez-Vendrell, Xavier
dc.contributor.author Jiménez, Alfons
dc.contributor.author Vásquez, Ana
dc.contributor.author Campillo, Ana
dc.contributor.author Incardona, Sandra
dc.contributor.author González, Raquel
dc.contributor.author Gamboa Vilela, Dionicia Baziliza
dc.contributor.author Torres Fajardo, Katherine Jessica
dc.contributor.author Oyibo, Wellington
dc.contributor.author Faye, Babacar
dc.contributor.author Macete, Eusebio
dc.contributor.author Menéndez, Clara
dc.contributor.author Ding, Xavier C.
dc.contributor.author Mayor, Alfredo
dc.date.accessioned 2020-07-14T00:02:33Z
dc.date.available 2020-07-14T00:02:33Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/20.500.12866/8337
dc.description.abstract BACKGROUND: Malaria diagnostics by rapid diagnostic test (RDT) relies primarily on the qualitative detection of Plasmodium falciparum histidine-rich protein 2 (PfHRP2) and Plasmodium spp lactate dehydrogenase (pLDH). As novel RDTs with increased sensitivity are being developed and implemented as point of care diagnostics, highly sensitive laboratory-based assays are needed for evaluating RDT performance. Here, a quantitative suspension array technology (qSAT) was developed, validated and applied for the simultaneous detection of PfHRP2 and pLDH in a variety of biological samples (whole blood, plasma and dried blood spots) from individuals living in different endemic countries. RESULTS: The qSAT was specific for the target antigens, with analytical ranges of 6.8 to 762.8 pg/ml for PfHRP2 and 78.1 to 17076.6 pg/ml for P. falciparum LDH (Pf-LDH). The assay detected Plasmodium vivax LDH (Pv-LDH) at a lower sensitivity than Pf-LDH (analytical range of 1093.20 to 187288.5 pg/ml). Both PfHRP2 and pLDH levels determined using the qSAT showed to positively correlate with parasite densities determined by quantitative PCR (Spearman r = 0.59 and 0.75, respectively) as well as microscopy (Spearman r = 0.40 and 0.75, respectively), suggesting the assay to be a good predictor of parasite density. CONCLUSION: This immunoassay can be used as a reference test for the detection and quantification of PfHRP2 and pLDH, and could serve for external validation of RDT performance, to determine antigen persistence after parasite clearance, as well as a complementary tool to assess malaria burden in endemic settings. en_US
dc.language.iso eng
dc.publisher BioMed Central
dc.relation.ispartofseries Malaria Journal
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Histidine-rich protein 2 en_US
dc.subject Luminex en_US
dc.subject Malaria en_US
dc.subject Parasite lactate dehydrogenase en_US
dc.subject Quantitative suspension array technology en_US
dc.subject Rapid diagnostic test en_US
dc.title Quantification of malaria antigens PfHRP2 and pLDH by quantitative suspension array technology in whole blood, dried blood spot and plasma en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1186/s12936-019-3083-5
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.07
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.08
dc.relation.issn 1475-2875


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