Universidad Peruana Cayetano Heredia

CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos

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dc.contributor.author Kushawah, G.
dc.contributor.author Hernandez-Huertas, L.
dc.contributor.author Abugattas-Nuñez del Prado, J.
dc.contributor.author Martinez-Morales, J.R.
dc.contributor.author DeVore, M.L.
dc.contributor.author Hassan, H.
dc.contributor.author Moreno-Sanchez, I.
dc.contributor.author Tomas-Gallardo, L.
dc.contributor.author Diaz-Moscoso, A.
dc.contributor.author Monges, D.E.
dc.contributor.author Guelfo, J.R.
dc.contributor.author Theune, W.C.
dc.contributor.author Brannan, E.O.
dc.contributor.author Wang, W.
dc.contributor.author Corbin, T.J.
dc.contributor.author Moran, A.M.
dc.contributor.author Sánchez Alvarado, A.
dc.contributor.author Málaga Trillo, George Edward
dc.contributor.author Takacs, C.M.
dc.contributor.author Bazzini, A.A.
dc.contributor.author Moreno-Mateos, M.A.
dc.date.accessioned 2020-12-14T16:06:27Z
dc.date.available 2020-12-14T16:06:27Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/20.500.12866/8723
dc.description.abstract The development of mRNA knockdown technologies for use in vertebrate organisms such as zebrafish has been limited. Kushawah et al. establish CRISPR-RfxCas13d as an efficient, specific, cost-effective, and straightforward method for the systematic and tractable study of gene function in vivo during embryogenesis across a range of animal species. © 2020 Elsevier Inc. Early embryonic development is driven exclusively by maternal gene products deposited into the oocyte. Although critical in establishing early developmental programs, maternal gene functions have remained elusive due to a paucity of techniques for their systematic disruption and assessment. CRISPR-Cas13 systems have recently been employed to degrade RNA in yeast, plants, and mammalian cell lines. However, no systematic study of the potential of Cas13 has been carried out in an animal system. Here, we show that CRISPR-RfxCas13d (CasRx) is an effective and precise system to deplete specific mRNA transcripts in zebrafish embryos. We demonstrate that zygotically expressed and maternally provided transcripts are efficiently targeted, resulting in a 76% average decrease in transcript levels and recapitulation of well-known embryonic phenotypes. Moreover, we show that this system can be used in medaka, killifish, and mouse embryos. Altogether, our results demonstrate that CRISPR-RfxCas13d is an efficient knockdown platform to interrogate gene function in animal embryos. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Developmental Cell
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject CRISPR-Cas13 en_US
dc.subject embryogenesis en_US
dc.subject zebrafish en_US
dc.subject RNA targeting en_US
dc.subject knockdown en_US
dc.subject early development en_US
dc.subject Cas13d en_US
dc.subject MZT en_US
dc.subject medaka en_US
dc.subject killifish en_US
dc.title CRISPR-Cas13d Induces Efficient mRNA Knockdown in Animal Embryos en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.devcel.2020.07.013
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 1878-1551


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