Universidad Peruana Cayetano Heredia

Lipoarabinomannan antigenic epitope differences in tuberculosis disease subtypes

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dc.contributor.author Magni, R.
dc.contributor.author Rruga, F.
dc.contributor.author Alsaab, F.
dc.contributor.author Sharif, S.
dc.contributor.author Howard, M.
dc.contributor.author Espina, V.
dc.contributor.author Kim, B.
dc.contributor.author Lepene, B.
dc.contributor.author Lee, G.
dc.contributor.author Alayouni, M.A.
dc.contributor.author Steinberg, H.
dc.contributor.author Araujo, R.
dc.contributor.author Kashanchi, F.
dc.contributor.author Riccardi, F.
dc.contributor.author Morreira, S.
dc.contributor.author Araujo, A.
dc.contributor.author Poli, F.
dc.contributor.author Jaganath, D.
dc.contributor.author Semitala, F.C.
dc.contributor.author Worodria, W.
dc.contributor.author Andama, A.
dc.contributor.author Choudhary, A.
dc.contributor.author Honnen, W.J.
dc.contributor.author Petricoin, E.F., III
dc.contributor.author Cattamanchi, A.
dc.contributor.author Colombatti, R.
dc.contributor.author de Waard, J.H.
dc.contributor.author Oberhelman, R.
dc.contributor.author Pinter, A.
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Liotta, L.A.
dc.contributor.author Luchini, A.
dc.date.accessioned 2020-12-14T16:06:31Z
dc.date.available 2020-12-14T16:06:31Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/20.500.12866/8732
dc.description.abstract An accurate urine test for diverse populations with active tuberculosis could be transformative for preventing TB deaths. Urinary liporabinomannan (LAM) testing has been previously restricted to HIV co-infected TB patients. In this study we evaluate urinary LAM in HIV negative, pediatric and adult, pulmonary and extrapulmonary tuberculosis patients. We measured 430 microbiologically confirmed pretreatment tuberculosis patients and controls from Peru, Guinea Bissau, Venezuela, Uganda and the United States using three monoclonal antibodies, MoAb1, CS35, and A194, which recognize distinct LAM epitopes, a one-sided immunoassay, and blinded cohorts. We evaluated sources of assay variability and comorbidities (HIV and diabetes). All antibodies successfully discriminated TB positive from TB negative patients. ROAUC from the average of three antibodies’ responses was 0.90; 95% CI 0.87–0.93, 90% sensitivity, 73.5% specificity (80 pg/mL). MoAb1, recognizing the 5-methylthio-d-xylofuranose(MTX)-mannose(Man) cap epitope, performed the best, was less influenced by glycosuria and identified culture positive pediatric (N = 19) and extrapulmonary (N = 24) patients with high accuracy (ROAUC 0.87, 95% CI 0.77–0.98, 0.90 sensitivity 0.80 specificity at 80 pg/mL; ROAUC = 0.96, 95% CI 0.92–0.99, 96% sensitivity, 80% specificity at 82 pg/mL, respectively). The MoAb1 antibody, recognizing the MTX-Man cap epitope, is a novel analyte for active TB detection in pediatric and extrapulmonary disease. en_US
dc.language.iso eng
dc.publisher Springer Nature
dc.relation.ispartofseries Scientific Reports
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Diagnosis en_US
dc.subject Paediatric research en_US
dc.subject Translational research en_US
dc.title Lipoarabinomannan antigenic epitope differences in tuberculosis disease subtypes en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1038/s41598-020-70669-9
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.07
dc.relation.issn 2045-2322


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