Objective: Using mixed methods, we conducted an acceptability study of an existing eHealth couples-based HIV prevention intervention with 42 male couples in Lima, Peru. Methods: Via individual-level interviews, 90% of couples reported high acceptability of the intervention, and liked the agreement builder activity the most. Results: Three key themes emerged, indicating couples wanted: more information about HIV treatment as prevention; suggestions to strengthen their own and familial relationships; the intervention to be more accessible for use (i.e., mobile). Conclusions: Findings suggest adaptation of the eHealth intervention is warranted to help fill a current gap in Public Health services: a couples-based HIV prevention intervention.