Universidad Peruana Cayetano Heredia

An electrochemical biosensor for the detection of Mycobacterium tuberculosis DNA from sputum and urine samples

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dc.contributor.author Ramos-Sono, D.
dc.contributor.author Laureano, R.
dc.contributor.author Rueda, D.
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Rosa, A.L.
dc.contributor.author Ruiz, Jesús
dc.contributor.author León, Raúl
dc.contributor.author Sheen Cortavarria, Patricia
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.date.accessioned 2020-12-14T16:10:06Z
dc.date.available 2020-12-14T16:10:06Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/20.500.12866/8776
dc.description.abstract Tuberculosis (TB) is a major global public health problem with high mortality and morbidity. In low-middle income countries (LMIC) a large number of respiratory symptomatic cases that require TB screening per year demands more accurate, fast and affordable testing for TB diagnostics. Sputum smear is the initial screening test in LMICs, however, its sensitivity is limited in patients with low sputum bacilli load. The same limitation is observed in the currently available molecular tests. We designed, standardized and evaluated an electrochemical biosensor that detects the highly specific DNA insertion element 6110 (IS6110). A PCR amplified DNA product is hybridized on the surface of the working electrode built on FTO-Glass with immobilized specific DNA probes, after which cyclic voltammetry is performed with an Ag/AgCl reference electrode and a platinum counter electrode. The response of the sensor was measured by the ratio (cathodic peak current of the hybridized sensor) / (cathodic peak current of the non-hybridized sensor). We tested the biosensor, using positive hybridization control sequences, genomic DNA extracted from M. tuberculosis strains and sputum of TB patients, and extracted DNA from the urine of healthy controls spiked with M. tuberculosis DNA. This biosensor was effective for the detection of M. tuberculosis DNA with a detection limit of 16 fM in sputum sample and 1 fM in spiked urine samples. The low cost and the relatively brief duration of the assay make this an important TB screening tool in the fight against tuberculosis. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS ONE
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Tuberculosis en_US
dc.subject Mycobacterium tuberculosis en_US
dc.subject Biosensors en_US
dc.subject Sputum en_US
dc.subject Polymerase chain reaction en_US
dc.subject Urine en_US
dc.subject DNA hybridization en_US
dc.subject DNA extraction en_US
dc.title An electrochemical biosensor for the detection of Mycobacterium tuberculosis DNA from sputum and urine samples en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pone.0241067
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.07
dc.relation.issn 1932-6203


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