Diagnostic urinary cfDNA detected in human cystic echinococcosis

Scott, A.L.; García Lescano, Héctor Hugo; Sedano, Cesar; Enriquez, Raul; Shiff, C.J.; Cysticercosis Working Group in Peru; Toribio, Luz; Soto Becerra, Percy; Santivañez, Saul

dc.contributor.author	Toribio, Luz
dc.contributor.author	Santivañez, Saul
dc.contributor.author	Scott, A.L.
dc.contributor.author	Enriquez, Raul
dc.contributor.author	Sedano, Cesar
dc.contributor.author	Soto Becerra, Percy
dc.contributor.author	García Lescano, Héctor Hugo
dc.contributor.author	Shiff, C.J.
dc.contributor.author	Cysticercosis Working Group in Peru
dc.date.accessioned	2020-12-14T16:10:18Z
dc.date.available	2020-12-14T16:10:18Z
dc.date.issued	2020
dc.identifier.uri	https://hdl.handle.net/20.500.12866/8826
dc.description.abstract	Cystic echinococcosis (CE) is a major neglected tropical zoonotic disease caused by the tissue-dwelling larval stage of the cestode parasite Echinococcus granulosus. For individuals suspected of CE, the diagnostic standard is imaging using ultrasonography, X rays, or computed tomography. These resource-demanding and expensive procedures are rarely available in endemic rural areas where CE is most prevalent. There is a critical need for a new approach to identify CE patients so that they can be managed early in the course of their infection. This study reports on the results of a diagnostic approach that identifies E. granulosus-derived cell-free DNA (cfDNA) in the urine of CE patients. Utilizing PCR to amplify a fragment of a major tandem repeat element found in E. granulosus nuclear DNA, urine samples from all seven imaging-confirmed CE patients who harbored active liver cysts were positive. In addition, the urine samples from 2/4 patients who presented with non-viable/calcified liver cysts were also PCR positive for the repeat fragment. To our knowledge, this is the first report of using parasite cfDNA from urine to diagnose CE. This approach provides an easy to implement and cost-effective method to survey for the prevalence of E. granulosus in humans populations.	en_US
dc.language.iso	eng
dc.publisher	Elsevier
dc.relation.ispartofseries	Molecular and Biochemical Parasitology
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject	Diagnosis of hydatid cysts	en_US
dc.subject	Cell-free DNA	en_US
dc.subject	Hydatidosis	en_US
dc.subject	Urinary DNA	en_US
dc.subject	Echinococcus granulosus	en_US
dc.subject	Peru	en_US
dc.title	Diagnostic urinary cfDNA detected in human cystic echinococcosis	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.1016/j.molbiopara.2020.111314
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#1.06.03
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.07
dc.relation.issn	1872-9428