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Molecular epidemiology reveals low genetic diversity among cryptococcus neoformans isolates from people living with hiv in lima, peru, during the pre-haart era

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dc.contributor.author van de Wiele, N.
dc.contributor.author Neyra, E.
dc.contributor.author Firacative, C.
dc.contributor.author Gilgado, F.
dc.contributor.author Serena, C.
dc.contributor.author Bustamante, B.
dc.contributor.author Meyer, W.
dc.date.accessioned 2020-12-14T16:10:22Z
dc.date.available 2020-12-14T16:10:22Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/20.500.12866/8840
dc.description.abstract Cryptococcosis, a mycosis presenting mostly as meningoencephalitis, affecting predominantly human immunodeficiency virus (HIV)-infected people, is mainly caused by Cryptococcus neoformans. The genetic variation of 48 C. neoformans isolates, recovered from 20 HIV-positive people in Lima, Peru, during the pre-highly active antiretroviral therapy (HAART) era, was studied retrospectively. The mating type of the isolates was determined by PCR, and the serotype by agglutination and CAP59-restriction fragment length polymorphism (RFLP). Genetic diversity was assessed by URA5-RFLP, PCR-fingerprinting, amplified fragment length polymorphism (AFLP), and multilocus sequence typing (MLST). All isolates were mating type alpha, with 39 molecular type VNI, seven VNII, corresponding to C. neoformans var. grubii serotype A, and two VNIII AD hybrids. Overall, the cryptococcal population from HIV-positive people in Lima shows a low degree of genetic diversity. In most patients with persistent cryptococcal infection, the same genotype was recovered during the follow-up. In four patients with relapse and one with therapy failure, different genotypes were found in isolates from the re-infection and from the isolate recovered at the end of the treatment. In one patient, two genotypes were found in the first cryptococcosis episode. This study contributes data from Peru to the ongoing worldwide population genetic analysis of Cryptococcus. en_US
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartof urn:issn:2076-0817
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject UNAVAILABLE en_US
dc.title Molecular epidemiology reveals low genetic diversity among cryptococcus neoformans isolates from people living with hiv in lima, peru, during the pre-haart era en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3390/pathogens9080665
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.00 es_PE


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