Ecological divergence and hybridization of Neotropical Leishmania parasites

Berriman, M.; Sanders, M.; Garcia, L.; Cotton, J.A.; Maes, I.; Dujardin, J.-C.; Cupolillo, E.; Arévalo Zelada, Jorge Luis; Llanos Cuentas, Elmer Alejandro; Imamura, H.; Van den Broeck, F.; Cooper, S.; Jara, Marlene; Adaui, V.; Schnaufer, A.; Miles, M.; Savill, N.J.; Mateus, D.

dc.contributor.author	Van den Broeck, F.
dc.contributor.author	Savill, N.J.
dc.contributor.author	Imamura, H.
dc.contributor.author	Sanders, M.
dc.contributor.author	Maes, I.
dc.contributor.author	Cooper, S.
dc.contributor.author	Mateus, D.
dc.contributor.author	Jara, Marlene
dc.contributor.author	Adaui, V.
dc.contributor.author	Arévalo Zelada, Jorge Luis
dc.contributor.author	Llanos Cuentas, Elmer Alejandro
dc.contributor.author	Garcia, L.
dc.contributor.author	Cupolillo, E.
dc.contributor.author	Miles, M.
dc.contributor.author	Berriman, M.
dc.contributor.author	Schnaufer, A.
dc.contributor.author	Cotton, J.A.
dc.contributor.author	Dujardin, J.-C.
dc.date.accessioned	2020-12-14T16:10:23Z
dc.date.available	2020-12-14T16:10:23Z
dc.date.issued	2020
dc.identifier.uri	https://hdl.handle.net/20.500.12866/8841
dc.description.abstract	The tropical Andes are an important natural laboratory to understand speciation in many taxa. Here we examined the evolutionary history of parasites of the Leishmania braziliensis species complex based on whole-genome sequencing of 67 isolates from 47 localities in Peru. We first show the origin of Andean Leishmania as a clade of near-clonal lineages that diverged from admixed Amazonian ancestors, accompanied by a significant reduction in genome diversity and large structural variations implicated in host-parasite interactions. Within the Andean species, patterns of population structure were strongly associated with biogeographical origin. Molecular clock and ecological niche modeling suggested that the history of diversification of the Andean lineages is limited to the Late Pleistocene and intimately associated with habitat contractions driven by climate change. These results suggest that changes in forestation over the past 150,000 y have influenced speciation and diversity of these Neotropical parasites. Second, genome-scale analyses provided evidence of meiotic-like recombination between Andean and Amazonian Leishmania species, resulting in full-genome hybrids. The mitochondrial genome of these hybrids consisted of homogeneous uniparental maxicircles, but minicircles originated from both parental species. We further show that mitochondrial minicircles- but not maxicircles-show a similar evolutionary pattern to the nuclear genome, suggesting that compatibility between nuclearencoded mitochondrial genes and minicircle-encoded guide RNA genes is essential to maintain efficient respiration. By comparing full nuclear and mitochondrial genome ancestries, our data expand our appreciation on the genetic consequences of diversification and hybridization in parasitic protozoa.	en_US
dc.language.iso	eng
dc.publisher	National Academy of Sciences
dc.relation.ispartofseries	Proceedings of the National Academy of Sciences of the United States of America
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.rights.uri	https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject	speciation genomics	en_US
dc.subject	ecological speciation	en_US
dc.subject	population genomics	en_US
dc.subject	vector-borne disease	en_US
dc.subject	interspecific hybridization	en_US
dc.title	Ecological divergence and hybridization of Neotropical Leishmania parasites	en_US
dc.type	info:eu-repo/semantics/article
dc.identifier.doi	https://doi.org/10.1073/pnas.1920136117
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#1.06.13
dc.subject.ocde	https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn	1091-6490