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Dissemination of a multidrug resistant CTX-M-65 producer Salmonella enterica serovar Infantis clone between marketed chicken meat and children

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dc.contributor.author Martínez-Puchol, Sandra
dc.contributor.author Riveros Ramirez, Maribel Denise
dc.contributor.author Ruidias, Kenny
dc.contributor.author Granda, Ana
dc.contributor.author Ruiz-Roldán, Lidia
dc.contributor.author Zapata-Cachay, Cristhian
dc.contributor.author Ochoa Woodell, Theresa Jean
dc.contributor.author Pons, Maria J.
dc.contributor.author Ruiz, Joaquim
dc.date.accessioned 2021-04-13T20:50:58Z
dc.date.available 2021-04-13T20:50:58Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/20.500.12866/9125
dc.description.abstract The objective of the present study was to characterize Salmonella enterica serovar Infantis isolated from chicken meat determining their clonal relationships with S. Infantis isolated from children with diarrhea. Fifteen meat-recovered S. Infantis were analyzed. Susceptibility levels to 14 antibacterial agents, the presence of ESBL and that of inducible plasmid-mediated AmpC (i-pAmpC) were determined by phenotypical methods. The presence of ESBL and pAmpC was confirmed by PCR, and detected ESBL-encoding genes were sequenced and their transferability tested by conjugation. The presence of gyrA mutations as well as Class 1 integrons was determined by PCR. Clonal relationships were established by REP-PCR and RAPD. In addition, 25 clinical isolates of S. Infantis were included in clonality studies. All meat-recovered S. Infantis were MDR, showing resistance to ampicillin, nitrofurans and quinolones, while none was resistant to azithromycin, ceftazidime or imipenem. ESBL (bla(CTX-M-65)) and i-pAmpC (bla(DHA)) were detected in 2 and 5 isolates respectively (in one case concomitantly), with bla(CTX-M-65) being transferable through conjugation. In addition, 1 isolate presented a bla(SHV) gene. All isolates presented D(87)Y at GyrA, nalidixic acid active efflux pump and a Class 1 integron of ~1000 bp (aadA1). Clonal analysis showed that all isolates were related. Further they were identical to MDR bla(CTX-M-65)-producing S. Infantis isolates causing children diarrhea in Lima. The dissemination of MDR bla(CTX-M-65)-producing S. Infantis between marketed meat and children highlights a public health problem which needs be controlled at livestock level. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries International Journal of Food Microbiology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Antibiotic resistance en_US
dc.subject ESBL en_US
dc.subject Foodborne diseases en_US
dc.title Dissemination of a multidrug resistant CTX-M-65 producer Salmonella enterica serovar Infantis clone between marketed chicken meat and children en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.ijfoodmicro.2021.109109
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 1879-3460


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