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Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis

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dc.contributor.author Orrego Solano, Miguel Ángel
dc.contributor.author Verastegui Pimentel, Manuela Renee
dc.contributor.author Vasquez, Carlos M.
dc.contributor.author Koziol, Uriel
dc.contributor.author Laclette, Juan P.
dc.contributor.author García Lescano, Héctor Hugo
dc.contributor.author Nash, Theodore E.
dc.date.accessioned 2021-04-13T20:51:04Z
dc.date.available 2021-04-13T20:51:04Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/20.500.12866/9244
dc.description.abstract Racemose neurocysticercosis is an aggressive disease caused by the aberrant expansion of the cyst form of Taenia solium within the subarachnoid spaces of the human brain and spinal cord resulting in a mass effect and chronic inflammation. Although expansion is likely caused by the proliferation and growth of the parasite bladder wall, there is little direct evidence of the mechanisms that underlie these processes. Since the development and growth of cysts in related cestodes involves totipotential germinative cells, we hypothesized that the expansive growth of the racemose larvae is organized and maintained by germinative cells. Here, we identified proliferative cells expressing the serine/threonine-protein kinase plk1 by in situ hybridization. Proliferative cells were present within the bladder wall of racemose form and absent from the homologous tissue surrounding the vesicular form. Cyst proliferation in the related model species Taenia crassiceps (ORF strain) occurs normally by budding from the cyst bladder wall and proliferative cells were concentrated within the growth buds. Cells isolated from bladder wall of racemose larvae were established in primary cell culture and insulin stimulated their proliferation in a dose-dependent manner. These findings indicate that the growth of racemose larvae is likely due to abnormal cell proliferation. The different distribution of proliferative cells in the racemose larvae and their sensitivity to insulin may reflect significant changes at the cellular and molecular levels involved in their tumor-like growth. Parasite cell cultures offer a powerful tool to characterize the nature and formation of the racemose form, understand the developmental biology of T. solium, and to identify new effective drugs for treatment. en_US
dc.description.sponsorship Este trabajo fue financiado por Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica (CONCYTEC) – Banco Mundial, a través de su unidad ejecutora Contrato Fondo Nacional para el Desarrollo de la Ciencia, Tecnología e Innovación Tecnológica (FONDECYT) [E033-01- 08-2018-FONDECYT/Banco Mundial-Programas de Doctorado en Áreas Estratégicas y Generales]. es_PE
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Larvae en_US
dc.subject Bladder en_US
dc.subject Insulin en_US
dc.subject Cell cultures en_US
dc.subject Cancers and neoplasms en_US
dc.subject Primary cell culture en_US
dc.subject Inflammation en_US
dc.subject Parasitic intestinal diseases en_US
dc.title Identification and culture of proliferative cells in abnormal Taenia solium larvae: Role in the development of racemose neurocysticercosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0009303
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1935-2735


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