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Triazole-resistance in environmental aspergillus fumigatus in latin american and african countries

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dc.contributor.author Resendiz-Sharpe, A.
dc.contributor.author Dewaele, K.
dc.contributor.author Merckx, R.
dc.contributor.author Bustamante Rufino, Ana Beatriz
dc.contributor.author Vega-Gomez, M.C.
dc.contributor.author Rolon, M.
dc.contributor.author Jacobs, J.
dc.contributor.author Verweij, P.E.
dc.contributor.author Maertens, J.
dc.contributor.author Lagrou, K.
dc.date.accessioned 2021-05-18T21:44:17Z
dc.date.available 2021-05-18T21:44:17Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/20.500.12866/9407
dc.description.abstract Triazole-resistance has been reported increasingly in Aspergillus fumigatus. An international expert team proposed to avoid triazole monotherapy for the initial treatment of invasive aspergillosis in regions with >10% environmental-resistance, but this prevalence is largely unknown for most American and African countries. Here, we screened 584 environmental samples (soil) from urban and rural locations in Mexico, Paraguay, and Peru in Latin America and Benin and Nigeria in Africa for triazole-resistant A. fumigatus. Samples were screened using triazole-containing agars and confirmed as triazole-resistant by the European Committee on Antimicrobial Susceptibility Testing (EUCAST) broth dilution reference method. Isolates were further characterized by cyp51A sequencing and short-tandem repeat typing. Fungicide presence in samples was likewise determined. Among A. fumigatus positive samples, triazole-resistance was detected in 6.9% (7/102) of samples in Mexico, 8.3% (3/36) in Paraguay, 9.8% (6/61) in Peru, 2.2% (1/46) in Nigeria, and none in Benin. Cyp51A gene mutations were present in most of the triazole-resistant isolates (88%; 15/17). The environmentally-associated mutations TR34/L98H and TR46/Y121F/T289A were prevalent in Mexico and Peru, and isolates harboring these mutations were closely related. For the first time, triazole-resistant A. fumigatus was found in environmental samples in Mexico, Paraguay, Peru, and Nigeria with a prevalence of 7–10% in the Latin American countries. Our findings emphasize the need to establish triazole-resistance surveillance programs in these countries en_US
dc.language.iso eng
dc.publisher MDPI
dc.relation.ispartofseries Journal of Fungi
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Africa en_US
dc.subject Epidemiology en_US
dc.subject Environment en_US
dc.subject America en_US
dc.subject Antifungal resistance en_US
dc.subject Aspergillus fumigatus en_US
dc.title Triazole-resistance in environmental aspergillus fumigatus in latin american and african countries en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3390/jof7040292
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.10
dc.relation.issn 2309-608X


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