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Assessment of Plasmodium antigens and CRP in dried blood spots with multiplex malaria array

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dc.contributor.author Jang, I.K.
dc.contributor.author Aranda, S.
dc.contributor.author Barney, R.
dc.contributor.author Rashid, A.
dc.contributor.author Helwany, M.
dc.contributor.author Rek, J.C.
dc.contributor.author Arinaitwe, E.
dc.contributor.author Adrama, H.
dc.contributor.author Murphy, M.
dc.contributor.author Imwong, M.
dc.contributor.author Proux, S.
dc.contributor.author Haohankhunnatham, W.
dc.contributor.author Ding, X.C.
dc.contributor.author Nosten, F.
dc.contributor.author Greenhouse, B.
dc.contributor.author Gamboa Vilela, Dionicia Baziliza
dc.contributor.author Domingo, G.J.
dc.date.accessioned 2021-10-04T23:00:55Z
dc.date.available 2021-10-04T23:00:55Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/20.500.12866/9785
dc.description.abstract Dried blood spots (DBS) typically prepared on filter papers are an ideal sample type for malaria surveillance by offering easy and cost-effective methods in terms of sample collection, storage, and transport. The objective of this study was to evaluate the applicability of DBS with a commercial multiplex malaria assay, developed to concurrently measure Plasmodium antigens, histidine-rich protein 2 (HRP2), Plasmodium lactate dehydrogenase (pLDH), and a host inflammatory biomarker, C-reactive protein (CRP), in whole blood. The assay conditions were optimized for DBS, and thermal stability for measurement of Plasmodium antigens and CRP in dried blood were determined. Performance of the multiplex assay on matched DBS and whole blood pellet samples was also evaluated using the clinical samples. The results indicate the acceptable performance in multiplex antigen detection using DBS samples. At cutoff levels for DBS, with a diagnostic specificity with a lower 95% confidence bound > 92%, diagnostic sensitivities against polymerase chain reaction (PCR)–confirmed malaria for HRP2, Pf LDH, Pv LDH, and Pan LDH were 93.5%, 80.4%, 21.3%, and 55.6%, respectively. The half-life of pLDH was significantly less than that of HRP2 in thermal stability studies. Results with DBS samples collected from Peru indicate that the uncontrolled storage conditions of DBS can result in inaccurate reporting for infection with P. falciparum parasites with hrp2/3 deletions. With careful consideration that minimizing the unfavorable DBS storage environment is essential for ensuring integrity of heat-labile Plasmodium antigens, DBS samples can be used as an alternative to liquid whole blood to detect P. falciparum with hrp2/3 deletions in malaria surveillance en_US
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartofseries Journal of Parasitic Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Dried blood spot en_US
dc.subject HRP2 en_US
dc.subject Immunoassay en_US
dc.subject LDH en_US
dc.subject Malaria en_US
dc.subject Multiplex en_US
dc.title Assessment of Plasmodium antigens and CRP in dried blood spots with multiplex malaria array en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1007/s12639-020-01325-2
dc.relation.issn 0975-0703


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