Universidad Peruana Cayetano Heredia

Congenital Chagas disease in Santa Cruz Department, Bolivia, is dominated by Trypanosoma cruzi lineage V

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dc.contributor.author Sanchez, Leny
dc.contributor.author Messenger, Louisa A.
dc.contributor.author Bhattacharyya, Tapan
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Mayta, Holger
dc.contributor.author Colanzi, Rony
dc.contributor.author Bozo, Ricardo
dc.contributor.author Verastegui Pimentel, Manuela Renee
dc.contributor.author Miles, Michael A.
dc.contributor.author Bern, Caryn
dc.date.accessioned 2021-10-04T23:00:56Z
dc.date.available 2021-10-04T23:00:56Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/20.500.12866/9803
dc.description.abstract BACKGROUND: This study identified Trypanosoma cruzi discrete typing units (DTUs) in maternal and infant specimens collected from two hospitals in Bolivia, using conventional genotyping and DTU-specific serotyping. METHODS: Specimens from 142 mothers were used, including 24 seronegative and 118 seropositive individuals; 29 women transmitted T. cruzi to their infants. Maternal and infant parasite loads were determined by quantitative real-time PCR. Maternal sera were tested with an in-house parasite lysate ELISA and serotyped by a lineage-specific peptide ELISA, targeting the trypomastigote small surface antigen (TSSA). Trypanosoma cruzi genotypes in infected infants were determined by a triple PCR-RFLP assay. RESULTS: All infant specimens were genotyped as TcV. Maternal parasite loads and absorbance values by the lysate ELISA were significantly higher for transmitters compared with non-transmitters. Among seropositive mothers, 65.3% had positive results by the TSSA II/V/VI peptide ELISA. No significant difference in reactivity to TSSA II/V/VI was observed for transmitters compared with non-transmitters (79.3% vs 60.7%, respectively). CONCLUSIONS: Our findings reinforce the difficulty in obtaining sufficient sample numbers and parasite DNA to investigate the interaction between parasite genetics and the risk of congenital transmission and argue for the inclusion of DTU-specific serotyping in prospective studies en_US
dc.language.iso eng
dc.publisher Oxford University Press
dc.relation.ispartofseries Transactions of the Royal Society of Tropical Medicine and Hygiene
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Bolivia en_US
dc.subject Chagas disease en_US
dc.subject Trypanosoma cruzi en_US
dc.subject congenital transmission en_US
dc.subject DTUs en_US
dc.subject TSSA en_US
dc.title Congenital Chagas disease in Santa Cruz Department, Bolivia, is dominated by Trypanosoma cruzi lineage V en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1093/trstmh/trab089
dc.relation.issn 1878-3503


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