Universidad Peruana Cayetano Heredia

Evaluation of a point-of-care molecular detection device for Leishmania spp. and intercurrent fungal and mycobacterial organisms in Peruvian patients with cutaneous ulcers

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dc.contributor.author Kariyawasam, Ruwandi
dc.contributor.author Valencia, Braulio M.
dc.contributor.author Lau, Rachel
dc.contributor.author Shao, Eric
dc.contributor.author Thompson, Courtney A.
dc.contributor.author Stevens, Michael
dc.contributor.author Kincaid, Leah
dc.contributor.author Del Castillo, Ana Luz Quispe
dc.contributor.author Cruz-Arzapalo, Lloysi O.
dc.contributor.author Llanos Cuentas, Elmer Alejandro
dc.contributor.author Boggild, Andrea K.
dc.date.accessioned 2021-10-04T23:00:57Z
dc.date.available 2021-10-04T23:00:57Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/20.500.12866/9823
dc.description.abstract PURPOSE: Overlapping clinical features of cutaneous leishmaniasis (CL) with ulcers caused by fungi and mycobacteria necessitate confirmatory diagnostic testing. We evaluated a handheld battery-operated device for detection of CL and common fungal and mycobacterial causes of ulcers. METHODS: We validated Palm PCR™ for detection of common ulcerative skin pathogens using ATCC(®) reference and clinical strains of Leishmania, mycobacteria, and fungi in the lab and field. Amplified products were Sanger sequenced. Performance characteristics were calculated using conventional PCR as a reference standard. RESULTS: Palm PCR™ detected 100% of ATCC(®) strains of Leishmania, fungi, and mycobacteria, with sensitivity and specificity of 90% and 91.7%, respectively. In the field, the sensitivity for detection of Leishmania in patients with suspected CL was 100%. In 61% of CL patients, co-colonization with genera such as Malassezia, Aspergillus, Candida, and Cladosporium was detected. In 50% of CL patients with an inflammatory (secondarily infected) phenotype, detected fungal species had known associations with human cutaneous disease. CONCLUSIONS: Palm PCR™ performs comparably to conventional PCR for detection of Leishmania, fungi, and mycobacteria. This work has implications for the diagnostic approach to tropical ulcers, and has the potential to improve field detection of ulcerative pathogens in resource constrained areas en_US
dc.language.iso eng
dc.publisher Springer
dc.relation.ispartofseries Infection
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Peru en_US
dc.subject Mycobacterium en_US
dc.subject Fungi en_US
dc.subject Cutaneous leishmaniasis en_US
dc.subject Point of care diagnostics en_US
dc.subject Tropical ulcer en_US
dc.title Evaluation of a point-of-care molecular detection device for Leishmania spp. and intercurrent fungal and mycobacterial organisms in Peruvian patients with cutaneous ulcers en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1007/s15010-021-01673-y
dc.relation.issn 1439-0973


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