Universidad Peruana Cayetano Heredia

Identification of proteins associated with the formation of oral biofilms

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dc.contributor.author Millones-Gómez, P.A.
dc.contributor.author Amaranto, R.E.B.
dc.contributor.author Torres, D.J.M.
dc.contributor.author Calla-Poma, R.D.
dc.contributor.author Requena-Mendizabal, M.F.
dc.contributor.author Alvino-Vales, M.I.
dc.contributor.author Calla-Poma, R.
dc.date.accessioned 2021-10-04T23:00:58Z
dc.date.available 2021-10-04T23:00:58Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/20.500.12866/9840
dc.description.abstract Objective: To identify proteins associated with the formation of Streptococcus gordonii and Fusobacterium nucleatum biofilms. Material and Methods: Biofilms composed of two bacterial species, S. gordonii and F. nucleatum, were cultured for 1, 4, 7, and 10 days. The presence of both species was confirmed via amplification of the srtA and radD genes using real-time PCR. The concentrations of proteins associated with the biofilms and individual species were quantified using Western blotting. Results: The protein profiles of S. gordonii and F. nucleatum from individual cultures determined using one-dimensional electrophoresis revealed proteins found in S. gordonii and in F. nucleatum. Ct and reciprocal Ct values were determined for the exposed S. gordonii and F. nucleatum biofilms. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was detected in biofilms and F. nucleatum, whereas HSP40 protein was present only in biofilms after 7 and 10 days of formation. Conclusion: HSP40 was detected only in the formed biofilms; thus, HSP40 is an essential proteins for adhesion en_US
dc.language.iso eng
dc.publisher Association of Support to Oral Health Research
dc.relation.ispartofseries Pesquisa Brasileira em Odontopediatria e Clinica Integrada
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject acetone en_US
dc.subject adhesion en_US
dc.subject alcohol en_US
dc.subject Article en_US
dc.subject bacterial growth en_US
dc.subject bacterial strain en_US
dc.subject bacterium culture en_US
dc.subject biofilm en_US
dc.subject carboxymethylcellulose en_US
dc.subject centrifugation en_US
dc.subject colony formation en_US
dc.subject cytoplasm protein en_US
dc.subject Dental Plaque en_US
dc.subject diaminobenzidine en_US
dc.subject electrophoresis en_US
dc.subject Fusobacterium nucleatum en_US
dc.subject gene amplification en_US
dc.subject genomics en_US
dc.subject Genomics en_US
dc.subject glyceraldehyde 3 phosphate dehydrogenase (NADP) en_US
dc.subject glycerol en_US
dc.subject heat shock protein 40 en_US
dc.subject hydrogen peroxide en_US
dc.subject nonhuman en_US
dc.subject oligonucleotide en_US
dc.subject phosphate buffered saline en_US
dc.subject polyacrylamide gel electrophoresis en_US
dc.subject polymerase chain reaction en_US
dc.subject protein analysis en_US
dc.subject protein expression en_US
dc.subject protein fingerprinting en_US
dc.subject pyroxylin en_US
dc.subject real time polymerase chain reaction en_US
dc.subject saliva substitute en_US
dc.subject Streptococcus gordonii en_US
dc.subject tooth plaque en_US
dc.subject trypsin en_US
dc.subject ultrasound en_US
dc.subject Western blotting en_US
dc.title Identification of proteins associated with the formation of oral biofilms en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1590/pboci.2021.084
dc.relation.issn 1983-4632


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