Universidad Peruana Cayetano Heredia

Mortality from drug-resistant tuberculosis in high-burden countries comparing routine drug susceptibility testing with whole-genome sequencing: a multicentre cohort study

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dc.contributor.author Zürcher, K.
dc.contributor.author Reichmuth, M.L.
dc.contributor.author Ballif, M.
dc.contributor.author Loiseau, C.
dc.contributor.author Borrell, S.
dc.contributor.author Reinhard, M.
dc.contributor.author Skrivankova, V.
dc.contributor.author Hömke, R.
dc.contributor.author Sander, P.
dc.contributor.author Avihingsanon, A.
dc.contributor.author Abimiku, A.G.
dc.contributor.author Marcy, O.
dc.contributor.author Collantes, J.
dc.contributor.author Carter, E.J.
dc.contributor.author Wilkinson, R.J.
dc.contributor.author Cox, H.
dc.contributor.author Yotebieng, M.
dc.contributor.author Huebner, R.
dc.contributor.author Fenner, L.
dc.contributor.author Böttger, E.C.
dc.contributor.author Gagneux, S.
dc.contributor.author Egger, M.
dc.contributor.author International epidemiology Databases to Evaluate AIDS
dc.date.accessioned 2021-10-04T23:00:59Z
dc.date.available 2021-10-04T23:00:59Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/20.500.12866/9854
dc.description.abstract Background: Drug resistance threatens global tuberculosis control. We aimed to examine mortality in patients with tuberculosis from high-burden countries, according to concordance or discordance of results from drug susceptibility testing done locally and whole-genome sequencing (WGS). Methods: In this multicentre cohort study, we collected pulmonary Mycobacterium tuberculosis isolates and clinical data from individuals with tuberculosis from antiretroviral therapy programmes and tuberculosis clinics in Côte d'Ivoire, Democratic Republic of the Congo, Kenya, Nigeria, Peru, South Africa, and Thailand, stratified by HIV status and drug resistance. Sites tested drug susceptibility using routinely available methods. WGS was done on Illumina HiSeq 2500 in the USA and Switzerland, and TBprofiler was used to analyse the genomes. We included individuals aged 16 years or older with pulmonary tuberculosis (bacteriologically confirmed or clinically diagnosed). We analysed mortality in multivariable logistic regression models adjusted for sex, age, HIV status, history of tuberculosis, and sputum positivity. Findings: Between Sept 1, 2014, and July 4, 2016, of 634 patients included in our previous analysis, we included 582 patients with tuberculosis (median age 33 years [IQR 27–43], 225 [39%] women, and 247 [42%] HIV-positive). Based on WGS, 339 (58%) isolates were pan-susceptible, 35 (6%) monoresistant, 146 (25%) multidrug-resistant, and 24 (4%) pre-extensively drug-resistant (pre-XDR) or XDR. The analysis of mortality was based on 530 patients; 63 (12%) died and 77 (15%) patients received inappropriate treatment. Mortality ranged from 6% (18 of 310) in patients with pan-susceptible tuberculosis to 39% (nine of 23) in patients with pre-XDR or XDR tuberculosis. The adjusted odds ratio for mortality was 4·92 (95% CI 2·47–9·78) among undertreated patients, compared with appropriately treated patients. Interpretation: In seven countries with a high burden of tuberculosis, we observed discrepancies between drug resistance patterns obtained locally and WGS. The underdiagnosis of drug resistance resulted in inappropriate treatment and higher mortality. WGS can provide accurate and detailed drug resistance information required to improve the outcomes of drug-resistant tuberculosis in high-burden settings. Our results support WHO's call for point-of-care tests based on WGS. Funding: National Institutes of Allergy and Infectious Diseases, Swiss National Science Foundation, and Swiss National Center for Mycobacteria en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Lancet. Microbe
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Mortality en_US
dc.subject drug-resistant tuberculosis en_US
dc.subject high-burden countries en_US
dc.subject drug susceptibility en_US
dc.subject whole-genome sequencing en_US
dc.title Mortality from drug-resistant tuberculosis in high-burden countries comparing routine drug susceptibility testing with whole-genome sequencing: a multicentre cohort study en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/S2666-5247(21)00044-6
dc.relation.issn 2666-5247


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