Universidad Peruana Cayetano Heredia

Pharmacological validation of Solanum mammosum L. as an anti-infective agent: Role of solamargine

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dc.contributor.author Cabanillas Amado, Billy Joel
dc.contributor.author Chassagne, F.
dc.contributor.author Vásquez-Ocmín, P.
dc.contributor.author Tahrioui, A.
dc.contributor.author Chevalier, S.
dc.contributor.author Vansteelandt, M.
dc.contributor.author Triastuti, A.
dc.contributor.author Amasifuen Guerra, C.A.
dc.contributor.author Fabre, N.
dc.contributor.author Haddad, M.
dc.date.accessioned 2021-10-04T23:00:59Z
dc.date.available 2021-10-04T23:00:59Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/20.500.12866/9869
dc.description.abstract Ethnopharmacological relevance: Fungal and bacterial infections remain a major problem worldwide, requiring the development of effective therapeutic strategies. Solanum mammosum L. (Solanaceae) (“teta de vaca”) is used in traditional medicine in Peru to treat fungal infections and respiratory disorders via topical application. However, the mechanism of action remains unknown, particularly in light of its chemical composition. Materials and methods: The antifungal activity of TDV was determined against Trichophyton mentagrophytes and Candida albicans using bioautography-TLC-HRMS to rapidly identify the active compounds. Then, the minimum inhibitory concentration (MIC) of the fruit crude extract and the active compound was determined to precisely evaluate the antifungal activity. Additionally, the effects of the most active compound on the formation of Pseudomonas aeruginosa biofilms and pyocyanin production were evaluated. Finally, a LC-HRMS profile and a molecular network of TDV extract were created to characterize the metabolites in the fruits' ethanolic extract. Results: Bioautography-TLC-HRMS followed by isolation and confirmation of the structure of the active compound by 1D and 2D NMR allowed the identification solamargine as the main compound responsible for the anti-Trichophyton mentagrophytes (MIC = 64 μg mL−1) and anti-Candida albicans (MIC = 64 μg mL−1) activities. In addition, solamargine led to a significant reduction of about 20% of the Pseudomonas aeruginosa biofilm formation. This effect was observed at a very low concentration (1.6 μg mL−1) and remained fairly consistent regardless of the concentration. In addition, solamargine reduced pyocyanin production by about 20% at concentrations of 12.5 and 50 μg mL−1. Furthermore, the LC-HRMS profiling of TDV allowed us to annotate seven known compounds that were analyzed through a molecular network. Conclusions: Solamargine has been shown to be the most active compound against T. mentoagrophytes and C. albicans in vitro. In addition, our data show that this compound affects significantly P. aeruginosa pyocyanin production and biofilm formation in our conditions. Altogether, these results might explain the traditional use of S. mammosum fruits to treat a variety of fungal infections and respiratory disorders en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Journal of Ethnopharmacology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Bioautography en_US
dc.subject Candida albicans en_US
dc.subject LC-HRMS en_US
dc.subject Pseudomonas aeruginosa en_US
dc.subject Solamargine en_US
dc.subject Solanum mammosum en_US
dc.subject Trichophyton mentagrophytes en_US
dc.title Pharmacological validation of Solanum mammosum L. as an anti-infective agent: Role of solamargine en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.jep.2021.114473
dc.relation.issn 1872-7573


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