dc.contributor.author |
D'Aeth, Joshua C. |
|
dc.contributor.author |
van der Linden, Mark Pg |
|
dc.contributor.author |
McGee, Lesley |
|
dc.contributor.author |
de Lencastre, Herminia |
|
dc.contributor.author |
Turner, Paul |
|
dc.contributor.author |
Song, Jae-Hoon |
|
dc.contributor.author |
Lo, Stephanie W. |
|
dc.contributor.author |
Gladstone, Rebecca A. |
|
dc.contributor.author |
Sá-Leão, Raquel |
|
dc.contributor.author |
Ko, Kwan Soo |
|
dc.contributor.author |
Hanage, William P. |
|
dc.contributor.author |
Breiman, Robert F. |
|
dc.contributor.author |
Beall, Bernard |
|
dc.contributor.author |
Bentley, Stephen D. |
|
dc.contributor.author |
Croucher, Nicholas J. |
|
dc.date.accessioned |
2021-10-04T23:01:01Z |
|
dc.date.available |
2021-10-04T23:01:01Z |
|
dc.date.issued |
2021 |
|
dc.identifier.uri |
https://hdl.handle.net/20.500.12866/9902 |
|
dc.description.abstract |
Multidrug-resistant Streptococcus pneumoniae emerge through the modification of core genome loci by interspecies homologous recombinations, and acquisition of gene cassettes. Both occurred in the otherwise contrasting histories of the antibiotic-resistant S. pneumoniae lineages PMEN3 and PMEN9. A single PMEN3 clade spread globally, evading vaccine-induced immunity through frequent serotype switching, whereas locally circulating PMEN9 clades independently gained resistance. Both lineages repeatedly integrated Tn916-type and Tn1207.1-type elements, conferring tetracycline and macrolide resistance, respectively, through homologous recombination importing sequences originating in other species. A species-wide dataset found over 100 instances of such interspecific acquisitions of resistance cassettes and flanking homologous arms. Phylodynamic analysis of the most commonly sampled Tn1207.1-type insertion in PMEN9, originating from a commensal and disrupting a competence gene, suggested its expansion across Germany was driven by a high ratio of macrolide-to-β-lactam consumption. Hence, selection from antibiotic consumption was sufficient for these atypically large recombinations to overcome species boundaries across the pneumococcal chromosome |
en_US |
dc.language.iso |
eng |
|
dc.publisher |
eLife Sciences Publications |
|
dc.relation.ispartofseries |
eLife |
|
dc.rights |
info:eu-repo/semantics/restrictedAccess |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
|
dc.subject |
epidemiology |
en_US |
dc.subject |
genetics |
en_US |
dc.subject |
infectious disease |
en_US |
dc.subject |
AMR |
en_US |
dc.subject |
genomics |
en_US |
dc.subject |
microbiology |
en_US |
dc.subject |
recombination |
en_US |
dc.subject |
streptococcus pneumoniae |
en_US |
dc.title |
The role of interspecies recombination in the evolution of antibiotic-resistant pneumococci |
en_US |
dc.type |
info:eu-repo/semantics/article |
|
dc.identifier.doi |
https://doi.org/10.7554/eLife.67113 |
|
dc.relation.issn |
2050-084X |
|