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Frecuencia de genes que codifican proteínas autotransportadoras serinproteasa de Enterobacteriaceae (SPATE) en cepas de Escherichia coli difusamente adherente (DAEC) provenientes de niños con y sin diarrea

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dc.contributor.author Ocampo, Karen
dc.contributor.author Riveros Ramirez, Maribel Denise
dc.contributor.author Pinedo-Bardales, Maria
dc.contributor.author Ruiz, Joaquim
dc.contributor.author Ochoa Woodell, Theresa Jean
dc.date.accessioned 2021-10-04T23:01:02Z
dc.date.available 2021-10-04T23:01:02Z
dc.date.issued 2021
dc.identifier.uri https://hdl.handle.net/20.500.12866/9924
dc.description.abstract The aim of the study was to determine the frequency of six genes encoding serine protease autotransporter proteins Enterobacteriaceae (SPATE) in diffusely adherent Escherichia coli (DAEC) isolates from children with (WD, n=63) and without diarrhea (WOD, n=41) from Lima, Peru. WOD were considered a control group. For the detection of the genes, 2 multiple PCRs were standardized: triple A (sigA, pet, espP) and triple B (sat, pic, espC). In both groups, the most frequent SPATE gene was Sat (39.7% of WD and 41.5% of WOD), followed by spP (20.6% and 9.7% in WD and WOD respectively). The other genes were detected in proportions lower than 10.0%, in the following order of frequency: pet, sigA, espC and pic, without significant differences between the groups. It was concluded that Sat is the most frequent SPATE in DAEC and that these strains may possess SPATE genes regardless of whether they are isolated in WD or WOD. en_US
dc.description.abstract El objetivo del estudio fue determinar la frecuencia de seis genes que codifican proteínas autotransportadoras serin-proteasa de Enterobacteriaceae (SPATE) en aislamientos de Escherichia coli difusamente adherente (DAEC) provenientes de niños con diarrea (NCD, n=63) y sin diarrea (NSD, n=41) de Lima, Perú. Los NSD se consideraron como grupo control. Para la detección de los genes se estandarizaron 2 PCRs múltiples: triple A (sigA, pet, espP) y triple B (sat, pic, espC). En ambos grupos el gen SPATE más frecuente fue sat (39,7% de NCD y 41,5% de NSD), seguido de espP (20,6% y 9,7% en NCD y NSD respectivamente). Los otros genes se detectaron en proporciones inferiores al 10,0%, en el siguiente orden de frecuencia: pet, sigA, espC y pic, sin diferencias significativas entre los grupos. Se concluye que Sat es la SPATE más frecuente en cepas DAEC, y que estas cepas pueden poseer genes SPATE independientemente de si se aíslan en NCD o NSD es_PE
dc.language.iso spa
dc.language.iso eng
dc.publisher Instituto Nacional de Salud
dc.relation.ispartofseries Revista Peruana de Medicina Experimental y Salud Pública
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Peru en_US
dc.subject Child en_US
dc.subject Diarrhea en_US
dc.subject Bacterial Toxins en_US
dc.subject Escherichia coli Infections en_US
dc.subject Escherichia coli Proteins/genetics en_US
dc.subject Enterobacteriaceae en_US
dc.subject Escherichia coli/genetics en_US
dc.subject Serine Proteases/genetics en_US
dc.subject Type V Secretion Systems en_US
dc.title Frecuencia de genes que codifican proteínas autotransportadoras serinproteasa de Enterobacteriaceae (SPATE) en cepas de Escherichia coli difusamente adherente (DAEC) provenientes de niños con y sin diarrea es_PE
dc.title.alternative Frequency of serine protease autotransporters of enterobacteriaceae (SPATE) encoding genes in diffusely adherent escherichia coli (DAEC) isolates from children with and without diarrhea en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.17843/rpmesp.2021.381.5750
dc.relation.issn 1726-4642


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