Listar por tema "DNA, Protozoan"

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  • Chromosomal copy number variation reveals differential levels of genomic plasticity in distinct Trypanosoma cruzi strains 
    Reis-Cunha, J.L.; Rodrigues-Luiz, G.F.; Valdivia, H.O.; Baptista, R.P.; Mendes, T.A.O.; Loss de Morais, G.; Guedes, R.; Macedo, A.M.; Bern, C.; Gilman, Robert Hugh; Talavera Lopez, C.; Andersson, B.; Vasconcelos, A.T.; Bartholomeu, D.C. (BioMed Central, 2015)
    Background: Trypanosoma cruzi, the etiologic agent of Chagas disease, is currently divided into six discrete typing units (DTUs), named TcI-TcVI. CL Brener, the reference strain of the T. cruzi genome project, is a hybrid ...
  • Comparative gene expression analysis throughout the life cycle of Leishmania braziliensis: Diversity of expression profiles among clinical isolates 
    Adaui, V.; Castillo Pareja, Denis Helan; Zimic-Peralta, Mirko Juan; Gutierrez, Andrés; Decuypere, S.; Vanaerschot, M.; de Doncker, S.; Schnorbusch, K.; Maes, I.; van der Auwera, G.; Maes, L.; Llanos Cuentas, Elmer Alejandro; Arévalo Zelada, Jorge Luis; Dujardin, J.-C. (Public Library of Science, 2011)
    Background: Most of the Leishmania genome is reported to be constitutively expressed during the life cycle of the parasite, with a few regulated genes. Inter-species comparative transcriptomics evidenced a low number of ...
  • Detection of Soluble Antigen and DNA of Trypanosoma cruzi in Urine Is Independent of Renal Injury in the Guinea Pig Model 
    Castro-Sesquen, Yagahira E.; Gilman, Robert Hugh; Yauri, Verónica; Cok, Jaime; Angulo, Noelia; Escalante, Hermes; Bern, Caryn (Public Library of Science, 2013)
    The diagnosis of Chagas disease in humans is generally limited to the detection of specific antibodies. Detection of T. cruzi antigens in urine has been reported previously, but is not used in the diagnosis. In this study, ...
  • Development of a novel protocol based on blood clot to improve the sensitivity of qPCR detection of toxoplasma gondii in peripheral blood specimens 
    Gutierrez Loli, Renzo Marcelo; Ferradas, C.; Diestra, A.; Traianou, A.; Bowman, N.; Bok, J.; Reimer-McAtee, M.; Ramal, C.; Ticona, E.; Steinberg, H.; Mayta, H.; Calderón Sánchez, Maritza Mercedes; Calla Choque, Jaeson Santos; Sterling, C.; Gilman, Robert Hugh; Pinedo, L.C.; Valencia, G.; Sanchez, L.; Málaga, E.; Zhu, D.; Jiménez, J.; Bern, C.; Angulo, N.; Schiaffino Salazar, Francesca; Acosta, J.; Holtz, M.; Clark, D.; Clark, T.; Trompeter, G.; Choi, J.; Gandarilla, O.; Dorn, M.; Fortuny, E.; Galdos, G.; Colanzi, R. (American Society of Tropical Medicine and Hygiene, 2019)
    Quantitative polymerase chain reaction (qPCR) for Toxoplasma gondii multicopy genes has emerged as a promising strategy for sensitive detection of parasite DNA. qPCR can be performed from blood samples, which are minimally ...
  • Diagnostic performance of filter paper lesion impression PCR for secondarily infected ulcers and nonulcerative lesions caused by cutaneous leishmaniasis 
    Boggild, A.K.; Ramos, A.P.; Valencia, B.M.; Veland, N.; Calderon, F.; Arévalo Zelada, Jorge Luis; Low, D.E.; Llanos Cuentas, Elmer Alejandro (American Society for Microbiology, 2011)
    We compared traditional cutaneous leishmaniasis diagnostic methods to filter paper lesion impression (FPLI) PCR for secondarily infected ulcers and nonulcerative lesions. The sensitivity and specificity of FPLI PCR for ...
  • Improved DNA extraction technique from clot for the diagnosis of Chagas disease 
    Mayta, Holger; Romero, Yomara K.; Pando, Alejandra; Verastegui Pimentel, Manuela Renee; Tinajeros, Freddy; Bozo, Ricardo; Henderson-Frost, Josephine; Colanzi, Rony; Flores, Jorge; Lerner, Richard; Bern, Caryn; Gilman, Robert Hugh (Public Library of Science, 2019)
    BACKGROUND: The detection of Trypanosoma cruzi genetic material in clinical samples is considered an important diagnostic tool for Chagas disease. We have previously demonstrated that PCR using clot samples yields greater ...
  • Isolation and characterization of Acanthamoeba strains from soil samples in Gran Canaria, Canary Islands, Spain 
    Reyes-Batlle, María; Todd, Cheridah D.; Martín-Navarro, Carmen M.; López-Arencibia, Atteneri; Cabello Vílchez, Alfonso Martín; González, Ana C.; Córdoba-Lanús, Elizabeth; Lindo, John F.; Valladares, Basilio; Piñero, José E.; Lorenzo-Morales, Jacob (Springer Verlag, 2014)
    Free-living Amoebae of Acanthamoeba genus include non-pathogenic and pathogenic strains that are currently classified in 18 different genotypes, T1-T18. In this study, a survey was carried out to evaluate the presence of ...
  • Isolation and molecular characterization of Acanthamoeba and Balamuthia mandrillaris from combination shower units in Costa Rica 
    Retana-Moreira, Lissette; Abrahams-Sandí, Elizabeth; Cabello Vílchez, Alfonso Martín; Reyes-Batlle, María; Valladares, Basilio; Martínez-Carretero, Enrique; Piñero, José E.; Lorenzo-Morales, Jacob (Springer Verlag, 2014)
    Free living amoebae (FLA) are ubiquitous protozoa, which may behave as parasites under certain conditions. Four genera are recognized as causal agents of infections in humans and animals: Naegleria, Sappinia, Acanthamoeba ...
  • Microgeographical differences of Plasmodium vivax relapse and re-infection in the Peruvian Amazon 
    Chuquiyauri, Raul; Peñataro, Pablo; Brouwer, Kimberly C.; Fasabi, Manuel; Calderón Sánchez, Maritza Mercedes; Torres, Sonia; Gilman, Robert Hugh; Kosek, Margaret; Vinetz, Joseph Michael (American Society of Tropical Medicine and Hygiene, 2013)
    To determine the magnitude of Plasmodium vivax relapsing malaria in rural Amazonia, we carried out a study in four sites in northeastern Peru. Polymerase chain reaction-restriction fragment length polymorphism of PvMSP-3α ...
  • Molecular epidemiology of plasmodium falciparum malaria outbreak, Tumbes, Peru, 2010–2012 
    Baldeviano, G.C.; Akinyi Okoth, S.; Arrospide, N.; Gonzalez, R.V.; Sánchez, J.F.; Macedo, S.; Conde, S.; Tapia, L.L.; Salas, C.; Gamboa Vilela, Dionicia Baziliza; Herrera, Y.; Edgel, K.A.; Udhayakumar, V.; Lescano Guevara, Andres Guillermo (Centers for Disease Control and Prevention, 2015)
    During 2010–2012, an outbreak of 210 cases of malaria occurred in Tumbes, in the northern coast of Peru, where no Plasmodium falciparum malaria case had been reported since 2006. To identify the source of the parasite ...
  • Patterns of selection on Plasmodium falciparum erythrocyte-binding antigens after the colonization of the New World 
    Yalcindag, Erhan; Rougeron, Virginie; Elguero, Eric; Arnathau, Céline; Durand, Patrick; Brisse, Sylvain; Diancourt, Laure; Aubouy, Agnes; Becquart, Pierre; D'Alessandro, Umberto; Fontenille, Didier; Gamboa Vilela, Dionicia Baziliza; Maestre, Amanda; Ménard, Didier; Musset, Lisie; Noya, Oscar; Veron, Vincent; Wide, Albina; Carme, Bernard; Legrand, Eric; Chevillon, Christine; Ayala, Francisco J.; Renaud, Francois; Prugnolle, Franck (Wiley, 2014)
    Pathogens, which have recently colonized a new host species or new populations of the same host, are interesting models for understanding how populations may evolve in response to novel environments. During its colonization ...
  • Whole genome sequencing analysis of Plasmodium vivax using whole genome capture 
    Bright, A.T.; Tewhey, R.; Abeles, S.; Chuquiyauri, R.; Llanos Cuentas, Elmer Alejandro; Ferreira, M.U.; Schork, N.J.; Vinetz, Joseph Michael; Winzeler, E.A. (BioMed Central, 2012)
    Background: Malaria caused by Plasmodium vivax is an experimentally neglected severe disease with a substantial burden on human health. Because of technical limitations, little is known about the biology of this important ...

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