Universidad Peruana Cayetano Heredia

Cavia porcellus as a model for experimental infection by trypanosoma cruzi

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dc.contributor.author Castro-Sesquen, Y.E.
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Yauri, V.
dc.contributor.author Angulo, N.
dc.contributor.author Verastegui Pimentel, Manuela Renee
dc.contributor.author Velásquez, D.E.
dc.contributor.author Sterling, C.R.
dc.contributor.author Martin, D.
dc.contributor.author Bern, C.
dc.date.accessioned 2022-01-18T19:26:45Z
dc.date.available 2022-01-18T19:26:45Z
dc.date.issued 2011
dc.identifier.uri https://hdl.handle.net/20.500.12866/10840
dc.description.abstract The guinea pig (Cavia porcellus) is a natural reservoir for Trypanosoma cruzi but has seldom been used as an experimental infection model. We developed a guinea pig infection model for acute and chronic Chagas disease. Seventy-two guinea pigs were inoculated intradermally with 104 trypomastigotes of T. cruzi strain Y (experimental group); 18 guinea pigs were used as control group. Eight animals from the experimental group and two from the control group were sacrificed 5, 15, 20, 25, 40, 55, 115, 165, and 365 days after inoculation. During the acute phase (15 to 55 days), we observed parasitemia (with a peak on day 20) and positive IgM and IgG Western blots with anti-shed acute-phase antigen bands. The cardiac tissue showed vasculitis, necrosis (on days 40 to 55), moderate to severe inflammation, and abundant amastigote nests. Smaller numbers of amastigote nests were also present in kidney, brain, and other organs. In the early chronic phase (115 to 165 days), parasitemia disappeared and antiT. cruzi IgG antibodies were still detectable. In cardiac tissue, the number of amastigote nests and the grade of inflammation decreased. In the chronic phase (365 days), the cardiac tissue showed vasculitis and fibrosis; detectable parasite DNA was associated with higher grades of inflammation. The experimental T. cruzi infection model in guinea pigs shows kinetics and pathologic changes similar to those of the human disease. en_US
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries American Journal of Pathology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Controlled Study en_US
dc.subject Animal Experiment en_US
dc.subject Disease Severity en_US
dc.subject Inflammation en_US
dc.subject Chronic Disease en_US
dc.subject Acute-Phase Reaction en_US
dc.subject Amastigote en_US
dc.subject Blotting Western en_US
dc.subject Cavia Porcellus en_US
dc.subject Chagas Disease en_US
dc.subject Disease Models |Enzyme-Linked Immunosorbent Assay en_US
dc.subject Experimental Infection en_US
dc.subject Fibrosis en_US
dc.subject Guinea Pigs en_US
dc.subject Immunoglobulin G Antibody en_US
dc.subject Immunoglobulin M Antibody en_US
dc.subject Parasitemia en_US
dc.subject Reverse Transcriptase Polymerase Chain Reaction en_US
dc.subject Trypanosoma Cruzi en_US
dc.subject Trypomastigote en_US
dc.subject Vasculitis en_US
dc.subject Vasculitis en_US
dc.subject Western Blotting en_US
dc.title Cavia porcellus as a model for experimental infection by trypanosoma cruzi en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.ajpath.2011.03.043
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.09
dc.relation.issn 1525-2191


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