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Alternative cost-effective media to facilitate MODS culture for diagnostics of tuberculosis

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dc.contributor.author Sheen Cortavarria, Patricia
dc.contributor.author Rodriguez, Joseline
dc.contributor.author Alcantara, Roberto
dc.contributor.author Vargas, Johnny
dc.contributor.author Grandjean, Louis
dc.contributor.author Moore, David Alexander James
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.date.accessioned 2022-11-15T23:04:38Z
dc.date.available 2022-11-15T23:04:38Z
dc.date.issued 2022
dc.identifier.uri https://hdl.handle.net/20.500.12866/12543
dc.description.abstract Most culture-based methods for tuberculosis diagnosis remain low-cost options for low- and mid-income countries. The MODS culture is a rapid and low-cost assay to diagnose tuberculosis and determine drug susceptibility. However, its implementation is limited due to the low accessibility to supplies required for the enriched medium. In this study, we evaluate two alternative culture media: A powder-based mixed (PM) and a lyophilized media (LM). Catalase, PANTA, and gamma irradiation were evaluated as additions to PM and LM. The culture performance of the alternative media was compared with the standard MODS medium (MM) using Mycobacterium tuberculosis isolates and positive acid-fast smear sputum samples. Overall, no significant difference was observed in the bacterial growth between PM and LM with MM. However, PANTA and gamma irradiation combined reduced bacterial growth significantly in all media variants. A median positivity day of 6 ± 5 days was observed for sputum samples, regardless of the culture medium. The preliminary results show that the two variants culture media have a similar performance to the standard MODS medium. The powder-based media with PANTA (PM_P) showed a time-to-positivity and sensitivity similar to the standard MODS medium. It is the simplest to prepare and does not require any sterilization process. en_US
dc.description.sponsorship Este trabajo fue financiado por CONCYTEC [número de contrato 157-2018]. es_PE
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartofseries Tuberculosis
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Cost-effective media en_US
dc.subject MODS culture en_US
dc.subject tuberculosis en_US
dc.title Alternative cost-effective media to facilitate MODS culture for diagnostics of tuberculosis en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1016/j.tube.2022.102225
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.02.07
dc.relation.issn 1873-281X


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