Universidad Peruana Cayetano Heredia

Uso de partículas magnéticas en la purificación de anticuerpos IgM contra Taenia solium

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dc.contributor.author Perez, L. Agueda
dc.contributor.author Castillo Berrios, Yesenia
dc.contributor.author Espinoza Guerrero, Cindy
dc.contributor.author Toribio, Luz M.
dc.contributor.author Santos, Yesica
dc.contributor.author Martel, Kevin S.
dc.contributor.author Wilkins, P.P.
dc.contributor.author Bustos Palomino, Javier Arturo
dc.contributor.author García Lescano, Héctor Hugo
dc.contributor.author Castro-Sesquen, Y.E.
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Gonzalez Zariquiey, Armando Emiliano
dc.contributor.author Tsang, V.C.W.
dc.contributor.author Rodriguez, S.
dc.contributor.author Gonzalez, I.
dc.contributor.author Saavedra Pastor, Herbert
dc.contributor.author Sanchez, S.
dc.contributor.author Martinez, M.
dc.contributor.author Verastegui Pimentel, Manuela Renee
dc.contributor.author Zimic-Peralta, Mirko Juan
dc.contributor.author Santivañez, Saul
dc.contributor.author Mayta, Holger
dc.contributor.author Pajuelo Travezaño, Monica Jhenny
dc.contributor.author Arroyo Hurtado, Gianfranco
dc.contributor.author Lopez, M.T.
dc.contributor.author Gomez, L.
dc.contributor.author Vargas, A.
dc.contributor.author Gavidia, C.M.
dc.contributor.author Moyano, L.M.
dc.contributor.author Gamboa Morán, Ricardo
dc.contributor.author Muro Ecca, Claudio Alberto
dc.contributor.author Vichez, P.
dc.contributor.author O´neal, S.
dc.contributor.author Handali, S.
dc.contributor.author Noh, J.
dc.contributor.author Nash, T.E.
dc.contributor.author Mahanty, S.
dc.contributor.author Friedland, J.
dc.date.accessioned 2020-12-14T16:10:04Z
dc.date.available 2020-12-14T16:10:04Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/20.500.12866/8764
dc.description.abstract The use of L protein coupled magnetic particles for the concentration and purification of immunoglobulin M (mIgM) monoclonal antibodies against Taenia solium was evaluated. Three concentration methods and different elution times were evaluated and the ratio of particles to the ratio of mIgM was optimized. It is demonstrated that: 1) with the use of magnetic particles, a previous concentration of mIgM is not required, which reduces the manipulation of the antibodies and improves the recovery, 2) the use of a binding buffer can be omitted, since the pH of most cell culture supernatants are neutral, and 3) longer elution times (~ 45 minutes) are needed to increase recovery to a level greater than 80%. The study demonstrates that the use of L protein-coupled magnetic particles is a simple and efficient tool for mIgM concentration and purification. en_US
dc.language.iso spa
dc.language.iso eng
dc.publisher Instituto Nacional de Salud
dc.relation.ispartofseries Revista Peruana de Medicina Experimental y Salud Pública
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Purification en_US
dc.subject Concentration en_US
dc.subject Taenia solium en_US
dc.subject Cysticercosis en_US
dc.subject Monoclonal Antibodies en_US
dc.subject Peru en_US
dc.subject Purificación es_PE
dc.subject Concentración es_PE
dc.subject Taenia solium es_PE
dc.subject Cisticercosis es_PE
dc.subject Anticuerpos Monoclonales es_PE
dc.subject Perú es_PE
dc.title Uso de partículas magnéticas en la purificación de anticuerpos IgM contra Taenia solium es_PE
dc.title.alternative Use of magnetic particles in the purification of igm antibodies against taenia solium en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.17843/rpmesp.2020.371.4628
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.05
dc.relation.issn 1726-4642


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