dc.contributor.author |
de Oliveira Mendes, Tiago Antonio |
|
dc.contributor.author |
Reis Cunha, Joao Luís |
|
dc.contributor.author |
de Almeida Lourdes, Rodrigo |
|
dc.contributor.author |
Rodrigues Luiz, Gabriela Flávia |
|
dc.contributor.author |
Lemos, Lucas Dhom |
|
dc.contributor.author |
dos Santos, Ana Rita Rocha |
|
dc.contributor.author |
da Câmara, Antonia Cláudia Jácome |
|
dc.contributor.author |
da Cunha Galvão, Lúcia Maria |
|
dc.contributor.author |
Bern, Caryn |
|
dc.contributor.author |
Gilman, Robert Hugh |
|
dc.contributor.author |
Fujiwara, Ricardo Tostes |
|
dc.contributor.author |
Gazzinelli, Ricardo Tostes |
|
dc.contributor.author |
Bartholomeu, Daniella Castanheira |
|
dc.date.accessioned |
2022-01-04T20:31:48Z |
|
dc.date.available |
2022-01-04T20:31:48Z |
|
dc.date.issued |
2013 |
|
dc.identifier.uri |
https://hdl.handle.net/20.500.12866/10668 |
|
dc.description.abstract |
The factors influencing variation in the clinical forms of Chagas disease have not been elucidated; however, it is likely that the genetics of both the host and the parasite are involved. Several studies have attempted to correlate the T. cruzi strains involved in infection with the clinical forms of the disease by using hemoculture and/or PCR-based genotyping of parasites from infected human tissues. However, both techniques have limitations that hamper the analysis of large numbers of samples. The goal of this work was to identify conserved and polymorphic linear B-cell epitopes of T. cruzi that could be used for serodiagnosis and serotyping of Chagas disease using ELISA. |
en_US |
dc.language.iso |
eng |
|
dc.publisher |
Public Library of Science |
|
dc.relation.ispartofseries |
PLoS Neglected Tropical Diseases |
|
dc.rights |
info:eu-repo/semantics/restrictedAccess |
|
dc.rights.uri |
https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es |
|
dc.subject |
Animals |
en_US |
dc.subject |
haplotype |
en_US |
dc.subject |
major clinical study |
en_US |
dc.subject |
controlled study |
en_US |
dc.subject |
enzyme linked immunosorbent assay |
en_US |
dc.subject |
Chagas disease |
en_US |
dc.subject |
Sensitivity and Specificity |
en_US |
dc.subject |
Trypanosoma cruzi |
en_US |
dc.subject |
animal model |
en_US |
dc.subject |
Mice |
en_US |
dc.subject |
mouse |
en_US |
dc.subject |
real time polymerase chain reaction |
en_US |
dc.subject |
DNA sequence |
en_US |
dc.subject |
genotype |
en_US |
dc.subject |
high throughput screening |
en_US |
dc.subject |
protein expression |
en_US |
dc.subject |
Antigens, Protozoan |
en_US |
dc.subject |
amino acid sequence |
en_US |
dc.subject |
B lymphocyte |
en_US |
dc.subject |
serodiagnosis |
en_US |
dc.subject |
Enzyme-Linked Immunosorbent Assay |
en_US |
dc.subject |
immunoblotting |
en_US |
dc.subject |
Mice, Inbred C57BL |
en_US |
dc.subject |
Serologic Tests |
en_US |
dc.subject |
computer model |
en_US |
dc.subject |
DNA extraction |
en_US |
dc.subject |
epitope |
en_US |
dc.subject |
antigenicity |
en_US |
dc.subject |
Computational Biology |
en_US |
dc.subject |
Epitopes, B-Lymphocyte |
en_US |
dc.subject |
genetic strain |
en_US |
dc.subject |
cross reaction |
en_US |
dc.subject |
epitope mapping |
en_US |
dc.subject |
peptide synthesis |
en_US |
dc.subject |
Protein Array Analysis |
en_US |
dc.subject |
protein microarray |
en_US |
dc.subject |
RNA extraction |
en_US |
dc.subject |
serotyping |
en_US |
dc.subject |
SPOT synthesis |
en_US |
dc.title |
Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays |
en_US |
dc.type |
info:eu-repo/semantics/article |
|
dc.identifier.doi |
https://doi.org/10.1371/journal.pntd.0002524 |
|
dc.subject.ocde |
https://purl.org/pe-repo/ocde/ford#3.03.06 |
|
dc.relation.issn |
1935-2735 |
|