Universidad Peruana Cayetano Heredia

Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays

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dc.contributor.author de Oliveira Mendes, Tiago Antonio
dc.contributor.author Reis Cunha, Joao Luís
dc.contributor.author de Almeida Lourdes, Rodrigo
dc.contributor.author Rodrigues Luiz, Gabriela Flávia
dc.contributor.author Lemos, Lucas Dhom
dc.contributor.author dos Santos, Ana Rita Rocha
dc.contributor.author da Câmara, Antonia Cláudia Jácome
dc.contributor.author da Cunha Galvão, Lúcia Maria
dc.contributor.author Bern, Caryn
dc.contributor.author Gilman, Robert Hugh
dc.contributor.author Fujiwara, Ricardo Tostes
dc.contributor.author Gazzinelli, Ricardo Tostes
dc.contributor.author Bartholomeu, Daniella Castanheira
dc.date.accessioned 2022-01-04T20:31:48Z
dc.date.available 2022-01-04T20:31:48Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/20.500.12866/10668
dc.description.abstract The factors influencing variation in the clinical forms of Chagas disease have not been elucidated; however, it is likely that the genetics of both the host and the parasite are involved. Several studies have attempted to correlate the T. cruzi strains involved in infection with the clinical forms of the disease by using hemoculture and/or PCR-based genotyping of parasites from infected human tissues. However, both techniques have limitations that hamper the analysis of large numbers of samples. The goal of this work was to identify conserved and polymorphic linear B-cell epitopes of T. cruzi that could be used for serodiagnosis and serotyping of Chagas disease using ELISA. en_US
dc.language.iso eng
dc.publisher Public Library of Science
dc.relation.ispartofseries PLoS Neglected Tropical Diseases
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Animals en_US
dc.subject haplotype en_US
dc.subject major clinical study en_US
dc.subject controlled study en_US
dc.subject enzyme linked immunosorbent assay en_US
dc.subject Chagas disease en_US
dc.subject Sensitivity and Specificity en_US
dc.subject Trypanosoma cruzi en_US
dc.subject animal model en_US
dc.subject Mice en_US
dc.subject mouse en_US
dc.subject real time polymerase chain reaction en_US
dc.subject DNA sequence en_US
dc.subject genotype en_US
dc.subject high throughput screening en_US
dc.subject protein expression en_US
dc.subject Antigens, Protozoan en_US
dc.subject amino acid sequence en_US
dc.subject B lymphocyte en_US
dc.subject serodiagnosis en_US
dc.subject Enzyme-Linked Immunosorbent Assay en_US
dc.subject immunoblotting en_US
dc.subject Mice, Inbred C57BL en_US
dc.subject Serologic Tests en_US
dc.subject computer model en_US
dc.subject DNA extraction en_US
dc.subject epitope en_US
dc.subject antigenicity en_US
dc.subject Computational Biology en_US
dc.subject Epitopes, B-Lymphocyte en_US
dc.subject genetic strain en_US
dc.subject cross reaction en_US
dc.subject epitope mapping en_US
dc.subject peptide synthesis en_US
dc.subject Protein Array Analysis en_US
dc.subject protein microarray en_US
dc.subject RNA extraction en_US
dc.subject serotyping en_US
dc.subject SPOT synthesis en_US
dc.title Identification of Strain-Specific B-cell Epitopes in Trypanosoma cruzi Using Genome-Scale Epitope Prediction and High-Throughput Immunoscreening with Peptide Arrays en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1371/journal.pntd.0002524
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.06
dc.relation.issn 1935-2735


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