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The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: The effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats

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dc.contributor.author Gonzales Rengifo, Gustavo Francisco
dc.contributor.author Vasquez Apéstegui, Bertha Vanessa
dc.contributor.author Gasco Tantachuco, Manuel Enrique
dc.date.accessioned 2022-01-04T20:33:21Z
dc.date.available 2022-01-04T20:33:21Z
dc.date.issued 2013
dc.identifier.uri https://hdl.handle.net/20.500.12866/10776
dc.description.abstract Transillumination technique for assessment of stages of spermatogenic cycle is a useful tool for toxicological studies. This study was designed to determine the effect of two medicinal plants on spermatogenesis in male rats using the transillumination technique. For this, the effect of the combination of a fruit with highest content of ascorbic acid (Myrciaria dubia, camu camu) and extract of black maca (Lepidium meyenii) on seminiferous tubule stages scored by transillumination on intact tubules in adult male rats was assessed. Animals were treated during seven days with vehicle, black maca, camu camu or a mixture of black maca + camu camu and assessed for daily sperm production (DSP), stages of spermatogenic cycle as well as antioxidant activity and levels of flavonoids and polyphenols. Black maca increased stages of spermiation (VII–VIII) and mitosis of germ cells (IX–XI), whereas camu camu increased stages of mitosis (IX–XI) and meiosis (XII). Mixture of maca + camu camu increased stages of spermiation, mitosis and meiosis. All treatments increased DSP (p < 0.05) and epididymal sperm count (p < 0.05). Total polyphenols, flavonoids levels and antioxidant activity were higher in camu camu (p < 0.001) than in black maca. In conclusion, M. dubia (camu camu) has potential effects improving spermatogenesis and co-administered with maca increase stages of mitosis, meiosis and spermiation of the spermatogenic cycle as assessed by the transillumination technique. This technique is becoming increasingly a useful tool for assessment spermatogenesis. en_US
dc.description.sponsorship Este trabajo fue financiado por CONCYTEC [número de contrato 217-2009]. es_PE
dc.language.iso eng
dc.publisher Taylor and Francis
dc.relation.ispartofseries Toxicology Mechanisms and Methods
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Black maca en_US
dc.subject Camu Camu en_US
dc.subject sperm count en_US
dc.subject spermatogenesis en_US
dc.subject transillumination en_US
dc.title The transillumination technique as a method for the assessment of spermatogenesis using medicinal plants: The effect of extracts of black maca (Lepidium meyenii) and camu camu (Myrciaria dubia) on stages of the spermatogenic cycle in male rats en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.3109/15376516.2013.802830
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.01.07
dc.relation.issn 1537-6524


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