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Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach

Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach

Veland, N.; Boggild, A.K.; Valencia, C.; Valencia, B.M.; Llanos Cuentas, Elmer Alejandro; Van Der Auwera, G.; Dujardin, J.-C.; Arévalo Zelada, Jorge Luis
URI: https://hdl.handle.net/20.500.12866/10999
Date: 2012

Abstract:

We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step.

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