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Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach

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dc.contributor.author Veland, N.
dc.contributor.author Boggild, A.K.
dc.contributor.author Valencia, C.
dc.contributor.author Valencia, B.M.
dc.contributor.author Llanos Cuentas, Elmer Alejandro
dc.contributor.author Van Der Auwera, G.
dc.contributor.author Dujardin, J.-C.
dc.contributor.author Arévalo Zelada, Jorge Luis
dc.date.accessioned 2022-01-18T19:26:53Z
dc.date.available 2022-01-18T19:26:53Z
dc.date.issued 2012
dc.identifier.uri https://hdl.handle.net/20.500.12866/10999
dc.description.abstract We present an algorithm based on three PCR assays for Leishmania (Viannia) species identification and assessed its performance using 70 specimens from Peruvian patients. The succession of the assayed targets can be ordered according to species prevalence. Sequential progression through the algorithm reduced the number of samples here studied by approximately 30% after each step. en_US
dc.language.iso eng
dc.publisher American Society for Microbiology
dc.relation.ispartofseries Journal of Clinical Microbiology
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Humans en_US
dc.subject Peru en_US
dc.subject article en_US
dc.subject major clinical study en_US
dc.subject priority journal en_US
dc.subject polymerase chain reaction en_US
dc.subject Leishmania braziliensis en_US
dc.subject Leishmaniasis, Cutaneous en_US
dc.subject Polymerase Chain Reaction en_US
dc.subject skin leishmaniasis en_US
dc.subject Algorithms en_US
dc.subject limit of detection en_US
dc.subject Leishmania en_US
dc.subject Leishmania lainsoni en_US
dc.subject DNA extraction en_US
dc.subject Viannia en_US
dc.subject algorithm en_US
dc.subject DNA en_US
dc.subject leishmania peruviana en_US
dc.subject analytic method en_US
dc.subject Parasitology en_US
dc.subject species identification en_US
dc.subject Molecular Diagnostic Techniques en_US
dc.subject sample en_US
dc.subject nucleic acid amplification en_US
dc.title Leishmania (Viannia) species identification on clinical samples from cutaneous leishmaniasis patients in Peru: Assessment of a molecular stepwise approach en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.1128/JCM.05061-11
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#1.06.01
dc.relation.issn 1098-660X


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