El objetivo del presente estudio fue evaluar el efecto remineralizante de una pasta dental experimental a base de nanohidroxiapatita y quitosano (nHAP/Q), como alternativa para favorecer la remineralización del esmalte dental bovino erosionado. Se evaluó el grado de mineralización mediante microscopia RAMAN y la microdureza superficial con Vickers. Se formuló una pasta dental base (PDB) que actuó como vehículo para los agentes remineralizantes en experimentación. Se elaboraron las siguientes pastas experimentales: PDB 80% + nHAp 10% + Quitosano 10%. (Grupo 1), PDB 90.%+ nHAp Concha de Abanico 10% (Grupo 2), PDB 90% + Quitosano 10%. (Grupo 3), PDB 90%+ nHAp comercial 10% (Grupo 4). Se establecieron como controles positivos 2 pastas dentales comerciales fluoradas de demostrada actividad remineralizante: Colgate total (Grupo 5) y Mi paste plus (Grupo 6). Se establecieron además los siguientes controles: PDB 100% (Grupo 7), Control ciclo erosivo, no pastas, conservación en saliva artificial (Grupo 8), Control no ciclo erosivo, no pastas (Grupo 9), Control ciclo erosivo, no pastas, conservación agua destilada (Grupo 10). Se establecieron 10 grupos de estudio con 10 muestras de esmalte de bovino cada uno, todos los especímenes de los grupos excepto del grupo 9 recibieron ataques con ácido cítrico al 0.5 % durante 2 min a las 0, 8, 24 y 32 horas. Después de cada ataque ácido, se aplicó las pastas de dientes correspondientes a cada grupo de tratamiento, excepto los grupos control 8, 9 y 10. Luego se procedió a evaluar el grado de mineralización mediante microscopia RAMAN y la microdureza superficial con Vickers. No encontramos diferencias significativas (p>0.05) en el grado de mineralización y Dureza superficial en el esmalte dental bovino, entre las diferentes pastas, las muestras de esmalte sano grupo 9) a las que no se les realizó ataque acido ni ningún tratamiento, ni las muestras del grupo 8 que recibieron el ataque ácido y fueron conservadas en saliva artificial. Pero si todas ellas, tienen diferencias significativas (p< 0.05) con el grupo 10 que fue sometido a ciclo erosivo y conservado en agua destilada. Este estudio in vitro muestra que la pasta experimental a base de nanohidroxiapatita y quitosano, tiene potencial remineralizante en el esmalte bovino erosionado.The objective of the present study was to evaluate the remineralizing effect of AN experimental toothpaste based on nanohydroxyapatite and chitosan (nHAP/Q), as an alternative to promote the remineralization of eroded bovine tooth enamel. The degree of mineralization was evaluated using RAMAN microscopy and the surface microhardness with Vickers. A base toothpaste (PDB) was formulated that acted as a vehicle for the remineralizing agents under experimentation. The following experimental pastes were prepared: PDB 80% + nHAp 10% + Chitosan 10% (Group 1), PDB 90%+ nHAp Argopten purpuratus Shell 10% (Group 2), PDB 90% + Chitosan 10% (Group 3), PDB 90%+ commercial nHAp 10% (Group 4). Two fluorinated commercial toothpastes with proven remineralizing activity were established as positive controls: Colgate total® (Group 5) and MI Paste PlusTM (Group 6). The following controls were also established: PDB 100% (Group 7), erosive cycle control, without pastes, and conserved in artificial saliva (Group 8), non-erosive cycle control, without pastes (Group 9), erosive cycle control, without pastes, conserved in distilled water (Group 10). 10 study groups were established with 10 bovine enamel samples each, all the specimens of all the groups except Group 9 received attacks with 0.5% citric acid for 2 min at 0, 8, 24 and 32 hours. After each acid attack, the toothpastes corresponding to each treatment group were applied, except for control groups 8, 9 and 10. The degree of mineralization was then evaluated using RAMAN microscopy and surface microhardness with Vickers. No significant differences (p>0.05) were found in the intensity of the phosphate ion using Raman v1, nor in the Vickers Hardness (kg/mm2) of bovine enamel, between the different pastes, the healthy enamel samples (group 9) towhich no acid attack or any treatment was carried out, nor the samples from group 8 that received the acid attack and were preserved in artificial saliva. But all of them have significant differences (p < 0.05) with group 10 that was subjected to an erosive cycle and preserved in distilled water. This in vitro study shows that, the experimental pastes based on nanohydroxyapatite and chitosan, has a potential remineralizing effect on eroded bovine tooth enamel.