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Resistencia a nitrofuranos en Salmonella enterica aisladas de carne para consumo humano

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dc.contributor.author Martínez-Puchol, Sandra
dc.contributor.author Pons, María J.
dc.contributor.author Ruiz-Roldán, Lidia
dc.contributor.author Laureano-Adame, Laura
dc.contributor.author Corujo, Alfredo
dc.contributor.author Ochoa Woodell, Theresa Jean
dc.contributor.author Ruiz, Joaquim
dc.date.accessioned 2020-07-14T00:02:33Z
dc.date.available 2020-07-14T00:02:33Z
dc.date.issued 2020
dc.identifier.uri https://hdl.handle.net/20.500.12866/8338
dc.description.abstract The mechanisms of resistance to nitrofurans from 18 meat samples with Salmonella enterica (chicken: 15; beef: 2; pork: 1) collected in Lima (Peru) were analyzed. The isolates were serotyped and the susceptibility levels to furazolidone and nitrofurantoin [with and without the efflux pump inhibitor Phenyl-Arginine-β-naphthylamide (PAβN)], the presence of mutations in the snrA and cnr genes and the transferability of resistance by conjugation were established. Fifteen samples with S. infantis (13 from chicken samples), 2 with S. enteritidis and 1 with S. anatum were identified. All isolates except the S. anatum were resistant to both nitrofurans showing MICs (minimum inhibitory concentration) of furazolidone and nitrofurantoin of 32-64 μg/mL and 128-256 μg/mL, respectively. The addition of PAßN had no effect on the MIC levels. All nitrofuran-resistant isolates showed amino acid codon alterations at both snrA and cnr (S. infantis: snrA STOP-151; cnr STOP-137; S. enteritidis: snrA STOP-180; cnr STOP-179). No transferable mechanisms of nitrofuran resistance were detected. en_US
dc.description.abstract En el presente estudio, se analizaron los mecanismos de resistencia a nitrofuranos en 18 muestras cárnicas con Salmonella enterica (15 de pollo, 2 de ternera y 1 de cerdo) de mercados de Lima (Perú). Determinaron los serotipos de los aislamientos y la sensibilidad a furazolidona y nitrofurantoina (con y sin el inhibidor de bombas de expulsión Phenyl-Arginine-β-Naphthylamide [PAβN]), las mutaciones en los genes snrA y cnr por PCR y la transferabilidad de la resistencia por conjugación. Se identificaron 15 muestras con S. infantis (13 muestras de pollo), 2 con S. enteritidis y 1 con S. anatum. Todos los aislamientos, excepto S. anatum, fueron resistentes a ambos nitrofuranos (concentración mínima inhibidora [CMI] a furazolidona: 32-64 μg/mL, CMI a nitrofurantoina: 128-256 μg/mL), sin diferencias al adicionarse PAβN. Todos los aislamientos resistentes a nitrofuranos presentaron sustituciones en snrA y cnr (S. infantis: snrA STOP-151; cnr STOP-137; S. enteritidis: snrA STOP-180; cnr STOP-179). No se detectaron mecanismos transferibles de resistencia a nitrofuranos es_PE
dc.language.iso spa
dc.language.iso eng
dc.publisher Instituto Nacional de Salud
dc.relation.ispartofseries Revista Peruana de Medicina Experimental y Salud Pública
dc.rights info:eu-repo/semantics/restrictedAccess
dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/deed.es
dc.subject Resistencia a Antibióticos es_PE
dc.subject Furazolidona es_PE
dc.subject Salmonella es_PE
dc.title Resistencia a nitrofuranos en Salmonella enterica aisladas de carne para consumo humano es_PE
dc.title.alternative Nitrofuran resistance in Salmonella enterica isolated from meat for human consumption en_US
dc.type info:eu-repo/semantics/article
dc.identifier.doi https://doi.org/10.17843/rpmesp.2020.371.4745
dc.subject.ocde https://purl.org/pe-repo/ocde/ford#3.03.05
dc.relation.issn 1726-4642


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